School of the Environment and Safety Engineering, Jiangsu University, No. 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, China.
School of the Environment and Safety Engineering, Jiangsu University, No. 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, China.
Chemosphere. 2018 Dec;212:768-776. doi: 10.1016/j.chemosphere.2018.08.136. Epub 2018 Aug 28.
Generally, biofilms developed for wastewater treatment readily detach from carrier medium once available thickness exceeds about 2 mm. Carrier media made of basalt fibers (BFs) could form ball-like aggregates (more than 10 cm in size, and called bio-nest). To demonstrate its feasibility for wastewater treatment, both reactors with and without BF carriers (RBF and RCO) were evaluated in terms of nutrient removal, oxygen mass transport and biological viabilities as well as biofilm adsorption characteristics. Therefore, oxygen microprofiles and confocal images for bio-nest as well as functional groups for biofilm-attached BF were performed on microsensor systems, confocal laser scanning microscopy (CLSM) and Fourier transform infrared (FTIR). Despite COD:N ratio, both reactors removed about 90% of COD, while only RBF reactor achieved high denitrification capabilities, with nitrogen removal efficiencies varying between 60.10 ± 0.45% and 82.07 ± 0.64%. Microprofile and confocal images showed that dissolved oxygen could reach the core with depth up to 50 mm, at which viable bacteria were detected. Characteristic peaks on FT-IR spectrum demonstrated that various functional groups of polysaccharide and proteins in EPS played a key role in aggregating biofilm-attached BFs into a bio-nest. Thus, BF provides a promising alternative to conventional carrier medium for wastewater treatment.
一般来说，一旦废水处理中生物膜的厚度超过 2 毫米，就很容易从载体上脱落。玄武岩纤维（BF）制成的载体可以形成球状聚集体（直径超过 10 厘米，称为生物巢）。为了证明其在废水处理中的可行性，评估了有无 BF 载体的两种反应器（RBF 和 RCO）在营养物质去除、氧气传质和生物活性以及生物膜吸附特性方面的性能。因此，在微传感器系统、共焦激光扫描显微镜（CLSM）和傅里叶变换红外（FTIR）上对生物巢的氧微剖面和共焦图像以及生物膜附着 BF 的官能团进行了研究。尽管 COD:N 比不同，但两种反应器都能去除约 90%的 COD，而只有 RBF 反应器具有较高的脱氮能力，其脱氮效率在 60.10 ± 0.45%至 82.07 ± 0.64%之间变化。微剖面和共焦图像表明，溶解氧可以到达 50 毫米深的核心区域，在那里检测到了存活的细菌。FT-IR 光谱上的特征峰表明，EPS 中各种多糖和蛋白质的官能团在将生物膜附着的 BF 聚集到生物巢中起着关键作用。因此，BF 为废水处理中的传统载体提供了一种有前途的替代方法。
