马传染性贫血病毒对犬胸腺细胞系的持续感染及病毒抗原产生的初步数据。

The persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens.

作者信息

Bouillant A M, Nielsen K, Ruckerbauer G M, Samagh B S, Hare W C

出版信息

J Virol Methods. 1986 Jul;13(4):309-21. doi: 10.1016/0166-0934(86)90056-x.

Abstract

Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluorescence. Chromosome analysis of EIAV-infected cells indicated they had a karyotype resembling the control cells of similar passage history. Virus-infected cells, grown in roller cultures for 65 days without subculturing, continuously produced viral antigens into supernatant fluids which were harvested every 3-4 days. Antigen peaks were observed at approximately 12-day intervals. Immunoprecipitin lines of identity were demonstrated between ether-extracted antigens from virus-infected canine cell line and known positive EIAV antigen extracted from infected equine spleen and a commercial source. Replication of a non-oncogenic retrovirus (EIAV) resulted in the continuous release of viral antigens from a persistently infected and infinite cell line.

摘要

马传染性贫血病毒（EIAV）被适应于Cf2Th细胞系，这是一种源自犬胸腺的异源恶性细胞系。通过在每第10代时用电子显微镜、免疫扩散和免疫荧光法证明病毒和病毒抗原的存在，对持续感染进行了100次连续传代监测。对EIAV感染细胞的染色体分析表明，它们的核型类似于具有相似传代历史的对照细胞。在旋转培养中生长65天未传代的病毒感染细胞，持续向上清液中产生病毒抗原，每3 - 4天收集一次上清液。大约每隔12天观察到抗原峰值。在从病毒感染的犬细胞系中提取的经乙醚处理的抗原与从感染马脾脏和商业来源提取的已知阳性EIAV抗原之间，证明了免疫沉淀线的一致性。一种非致癌逆转录病毒（EIAV）的复制导致从持续感染的无限细胞系中持续释放病毒抗原。