Whetter L, Archambault D, Perry S, Gazit A, Coggins L, Yaniv A, Clabough D, Dahlberg J, Fuller F, Tronick S
Department of Microbiology, Pathology & Parasitology, North Carolina State University, College of Veterinary Medicine, Raleigh 27606.
J Virol. 1990 Dec;64(12):5750-6. doi: 10.1128/JVI.64.12.5750-5756.1990.
A full-length molecular clone of equine infectious anemia virus (EIAV) was isolated from a persistently infected canine fetal thymus cell line (Cf2Th). Upon transfection of equine dermis cells, the clone, designated CL22, yielded infectious EIAV particles (CL22-V) that replicated in vitro in both Cf2Th cells and an equine dermis cell strain. Horses infected with CL22-V developed an antibody response to viral proteins and possessed viral DNA in peripheral blood mononuclear cells, as determined by polymerase chain reaction assays. In addition, horses infected with CL22-V became persistently infected and were capable of transmitting the infection by transfer of whole blood to uninfected horses. However, CL22-V, like the parental canine cell-adapted virus, did not cause clinical signs in infected horses. Reverse transcriptase assays of CL22-V- and virulent EIAV-infected equine mononuclear cell cultures indicated that the lack of virulence of CL22-V was not due to an inability to infect and replicate in equine mononuclear cells in vitro.
从持续感染的犬胎儿胸腺细胞系（Cf2Th）中分离出了马传染性贫血病毒（EIAV）的全长分子克隆。将该克隆转染至马真皮细胞后，命名为CL22的克隆产生了具有感染性的EIAV颗粒（CL22-V），其可在Cf2Th细胞和一种马真皮细胞系中进行体外复制。感染CL22-V的马匹对病毒蛋白产生了抗体反应，并且通过聚合酶链反应检测确定其外周血单核细胞中存在病毒DNA。此外，感染CL22-V的马匹持续感染，并能够通过将全血输给未感染的马匹来传播感染。然而，CL22-V与亲本犬细胞适应病毒一样，在感染的马匹中未引起临床症状。对CL22-V和强毒EIAV感染的马单核细胞培养物进行逆转录酶检测表明，CL22-V缺乏毒力并非由于其无法在体外感染马单核细胞并进行复制。
