Semsei I, Zs-Nagy I
J Free Radic Biol Med. 1985;1(5-6):403-8. doi: 10.1016/0748-5514(85)90153-9.
The superoxide radical scavenging ability of centrophenoxine (CPH) and its components (dimethylaminoethanol = DMAE, p-chlorophenoxyacetic acid = PCPA) was studied in vitro using the method of pyrogallol autoxidation, cytochrome c reduction and photoxidation of o-dianisidine in salt-free assay media and in the presence of increasing NaCl or KCl concentrations. The CPH proved to be a superoxide radical scavenger in all three systems used, however, the rate constant for this reaction was rather low (1.7 X 10(2) M-1 s-1). This scavenging ability decreased linearly with increasing ionic strength. DMAE and PCPA behaved in a somewhat contradictory manner. The former proved to be a weak superoxide radical generating compound being partially sensitive to the ionic strength. The latter showed either superoxide radical scavenging or generating effects in various assays depending on the actual salt concentrations of the media. On the basis of the results one has to assume that the superoxide radical scavenger ability of CPH may hardly be responsible for the in vivo effects of this compound, therefore, its OH. radical scavenger reactions the rate constant of which is about 10(9) M-1 s-1 may be of much greater importance.
在无盐测定介质中以及存在不断增加的氯化钠或氯化钾浓度的情况下，采用邻苯三酚自氧化法、细胞色素c还原法以及邻联茴香胺光氧化法，在体外研究了氯酯醒（CPH）及其组分（二甲氨基乙醇 = DMAE，对氯苯氧乙酸 = PCPA）的超氧阴离子自由基清除能力。在所有使用的三种体系中，CPH都被证明是一种超氧阴离子自由基清除剂，然而，该反应的速率常数相当低（1.7×10² M⁻¹ s⁻¹）。这种清除能力随离子强度的增加呈线性下降。DMAE和PCPA的表现有些矛盾。前者被证明是一种较弱的超氧阴离子自由基生成化合物，对离子强度部分敏感。后者在各种测定中根据介质的实际盐浓度表现出超氧阴离子自由基清除或生成作用。基于这些结果，人们不得不假定CPH的超氧阴离子自由基清除能力可能很难对该化合物的体内效应负责，因此，其羟基自由基清除反应（其速率常数约为10⁹ M⁻¹ s⁻¹）可能更为重要。
