Wu Chuan-Qin, Chen Guan-Hua
College of Food and Bioengineering, Jiangsu University, Zhenjiang, China.
Biomed Chromatogr. 2019 Oct;33(10):e4630. doi: 10.1002/bmc.4630. Epub 2019 Aug 1.
A new capillary electrophoresis method was developed to study the synergistic effect of superoxide dismutase and jujuboside A or B on scavenging superoxide anion radical in serum matrix respectively, in which superoxide anion radical was generated from pyrogallol autoxidation. The electrophoresis conditions, and the factors affecting the productive rate of purpurogallin, such as pyrogallol autoxidation product and the activity of superoxide dismutase, were optimized. Under optimal conditions, the content of superoxide dismutase in Gibco newborn calf serum was 7.06 mg/L, RSD was 2.01% and the average recovery was 98.4%. The values of IC for jujuboside A and B in the serum matrix were 157.67 and 31.60 mg/L respectively, and they both had synergy on scavenging superoxide anion radical with superoxide dismutase, but there was no the dose-dependency on this synergy.
建立了一种新的毛细管电泳方法，分别研究超氧化物歧化酶与枣仁皂苷A或B在血清基质中清除超氧阴离子自由基的协同作用，其中超氧阴离子自由基由邻苯三酚自氧化产生。优化了电泳条件以及影响邻苯三酚红生产率的因素，如邻苯三酚自氧化产物和超氧化物歧化酶的活性。在最佳条件下，Gibco新生牛血清中超氧化物歧化酶的含量为7.06 mg/L，相对标准偏差为2.01%，平均回收率为98.4%。血清基质中枣仁皂苷A和B的半数抑制浓度分别为157.67和31.60 mg/L，它们与超氧化物歧化酶在清除超氧阴离子自由基方面均具有协同作用，但这种协同作用不存在剂量依赖性。
