Parker N B, Berger E M, Curtis W E, Muldrow M E, Linas S L, Repine J E
J Free Radic Biol Med. 1985;1(5-6):415-9. doi: 10.1016/0748-5514(85)90155-2.
Addition of increasing concentrations of hydrogen peroxide (H2O2) caused progressive decreases in dimethylthiourea (DMTU) concentrations which were inhibitable by simultaneous addition of catalase, but not the superoxide anion (O2-.) scavenger, superoxide dismutase (SOD), or hydroxyl radical (.OH) scavengers, such as mannitol, sodium benzoate or dimethyl sulfoxide (DMSO). In parallel, addition of increasing concentrations of H2O2 with FE++/EDTA (but not H2O2 alone) caused decreases in DMSO concentrations which were inhibitable by simultaneous addition of .OH scavengers but not SOD or catalase. Addition of DMTU, but not DMSO, also decreased H2O2 concentrations in vitro. The results indicate the relative scavenging specificities of DMTU and DMSO for H2O2 and .OH, respectively. The findings also suggest that measurement of DMTU or DMSO consumption could help assess the contribution of O2 metabolites in biological systems.
添加浓度不断增加的过氧化氢（H2O2）会导致二甲基硫脲（DMTU）浓度逐渐降低，同时添加过氧化氢酶可抑制这种降低，但超氧阴离子（O2-.）清除剂超氧化物歧化酶（SOD）或羟基自由基（.OH）清除剂（如甘露醇、苯甲酸钠或二甲基亚砜（DMSO））则不能抑制。同时，添加浓度不断增加的H2O2与亚铁离子/乙二胺四乙酸（FE++/EDTA）（而非单独的H2O2）会导致DMSO浓度降低，同时添加.OH清除剂可抑制这种降低，但SOD或过氧化氢酶则不能。添加DMTU而非DMSO也会在体外降低H2O2浓度。结果表明DMTU和DMSO分别对H2O2和.OH具有相对清除特异性。这些发现还表明，测量DMTU或DMSO的消耗有助于评估生物系统中O2代谢产物的作用。
