Carlin G, Arfors K E
J Free Radic Biol Med. 1985;1(5-6):437-42. doi: 10.1016/0748-5514(85)90158-8.
Human polymorphonuclear leucocytes were found to promote peroxidation of phospholipid liposomes upon stimulation by phorbol myristate acetate. Peroxidation required the presence of either pyrophosphate-chelated or ADP-chelated iron, whereas iron chelated to EDTA or ATP had no effect. Peroxidation was also catalyzed by ferritin, but not by transferrin. Superoxide dismutase abolished the peroxidation, whereas catalase and apparently also the hydroxyl radical scavenger dimethyl sulphoxide were inactive, indicating that the peroxidation was mediated by superoxide radicals but not by hydrogen peroxide or hydroxyl radicals. Xanthine oxidase-promoted peroxidation was studied for comparison and showed similar characteristics except that transferrin catalyzed the peroxidation. Peroxidation of membrane lipids may be a mechanism whereby granulocytes cause tissue damage in inflammation. The drugs paracetamol, gentisic acid and 5-aminosalicylic acid inhibited lipid peroxidation, probably through their ability to react with the superoxide anion.
发现人类多形核白细胞在佛波酯肉豆蔻酸酯乙酸盐刺激下可促进磷脂脂质体的过氧化作用。过氧化作用需要焦磷酸螯合铁或ADP螯合铁的存在，而与EDTA或ATP螯合的铁则没有作用。铁蛋白也可催化过氧化作用，但转铁蛋白则不能。超氧化物歧化酶可消除过氧化作用，而过氧化氢酶以及显然还有羟基自由基清除剂二甲亚砜则无活性，这表明过氧化作用是由超氧自由基介导的，而非过氧化氢或羟基自由基。为作比较研究了黄嘌呤氧化酶促进的过氧化作用，其显示出相似的特征，只是转铁蛋白可催化过氧化作用。膜脂质的过氧化作用可能是粒细胞在炎症中造成组织损伤的一种机制。对乙酰氨基酚、龙胆酸和5-氨基水杨酸可抑制脂质过氧化作用，可能是通过它们与超氧阴离子反应的能力来实现的。
