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通过在拟南芥中生产 D-松醇提高盐和耐旱性。

Increased salt and drought tolerance by D-pinitol production in transgenic Arabidopsis thaliana.

机构信息

Department of Bioscience and Biotechnology, University of Suwon, Hwaseong, South Korea.

Department of Bioscience and Biotechnology, University of Suwon, Hwaseong, South Korea; Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh.

出版信息

Biochem Biophys Res Commun. 2018 Sep 26;504(1):315-320. doi: 10.1016/j.bbrc.2018.08.183. Epub 2018 Sep 1.

DOI:10.1016/j.bbrc.2018.08.183
PMID:30180952
Abstract

D-ononitol epimerase (OEP) catalyzes the conversion of D-ononitol to D-pinitol, which is the last step in the biosynthetic pathway, where myo-inositol is converted to pinitol in higher plants. In this study, OEP cDNA was isolated from Glycine max (GmOEP) and was functionally characterized, which confirmed that GmOEP expression was induced by high salinity and drought stress treatments. To understand the biological function of GmOEP, transgenic Arabidopsis plants overexpressing this protein were constructed. The transgenic Arabidopsis plants displayed enhanced tolerance to high salinity and drought stress treatments.

摘要

D-甘露醇差向异构酶(OEP)催化 D-甘露醇向 D-松醇的转化,这是生物合成途径的最后一步,在高等植物中,肌醇转化为松醇。在这项研究中,从大豆(GmOEP)中分离出 OEP cDNA 并进行了功能表征,证实 GmOEP 的表达受高盐和干旱胁迫处理诱导。为了了解 GmOEP 的生物学功能,构建了过量表达该蛋白的转基因拟南芥植物。转基因拟南芥植物对高盐和干旱胁迫处理表现出增强的耐受性。

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