Yura Y, Iga H, Terashima K, Yoshida H, Yanagawa T, Azuma M, Hayashi Y, Sato M
Int J Oral Maxillofac Surg. 1986 Aug;15(4):457-63. doi: 10.1016/s0300-9785(86)80038-2.
A 48-year-old female had primary herpetic gingivostomatitis, followed by recurrent intraoral herpes simplex virus (HSV) disease; HSV isolates were obtained from the swabs of primary and recurrent lesions; restriction endonuclease cleavage analysis of the viral DNAs extracted from Vero cells infected with the HSV isolates according to the method of Hirt was carried out. The viral DNAs were cleaved by restriction endonucleases such as BamHI, KpnI and SalI and resolved by agarose gel electrophoresis, followed by staining with ethidium bromide. Consequently, their cleavage patterns were very similar to one another and were identified as HSV type 1. From these findings, it can be concluded that primary and recurrent lesions of this case are caused by the same virus.
一名48岁女性患有原发性疱疹性龈口炎，随后出现复发性口腔单纯疱疹病毒（HSV）疾病；从原发性和复发性病变的拭子中获取HSV分离株；按照Hirt的方法，对从感染了HSV分离株的Vero细胞中提取的病毒DNA进行限制性内切酶切割分析。病毒DNA用BamHI、KpnI和SalI等限制性内切酶切割，通过琼脂糖凝胶电泳分离，然后用溴化乙锭染色。结果，它们的切割模式彼此非常相似，被鉴定为1型HSV。从这些发现可以得出结论，该病例的原发性和复发性病变由同一病毒引起。
