Nasrabadi Nafiseh Nasri, Sargazi Farshid, Shokrzadeh Mohammad, Abediankenari Saeid, Hoseini Seyed Vahid, Najafi Mojtaba, Haghi-Aminjan Hamed, Mirmajidi Seyedeh Habibeh, Ataee Ramin
Pharmaceutical Sciences Research Centre, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Pharmaceutical sciences research center, Mazandaran University of medical sciences, Sari, Iran.
Neuro Endocrinol Lett. 2018 May;39(2):111-118.
Gastric cancer accounts 8% of the total cancer cases leading to 10% of total cancer deaths worldwide. The indoleamine N-acetyl-5-methoxytryptamine, better known as melatonin, is the principal hormone produced by the pineal gland. Recently, it has been well documented some anti-cancer roles of melatonin in some malignancies as breast and colon cancer; as well as some its protective roles in the GI tract that have been known as free radical scavenger, antimitogenic and apoptotic properties. According to the anti-cancer effects of melatonin, wide distribution of this neurohormone in GI tract and some proposed physiologic and pharmacologic roles for this neurohormone and following our previous study which has shown expression of MT2 receptor in gastric adenocarcinoma, this study initially scheduled to determine the expression of melatonin receptor MT1 in tissue samples of adenocarcinoma cancer patients. A total of 10 gastric adenocarcinoma patients and 10 normal individuals were examined for MT1 gene expression by real-time PCR. Additionally, for screening of different alleles of MT1 in our samples, the SSCP-PCR procedure was developed. Our results have shown interestingly high expression for MT1 receptor in cancer and marginal cancer groups comparing with normal group. Our findings also have shown that a remarkable association between MT1 receptor mRNA levels and grade in individuals over age 50. PCR-SSCP analysis results showed a variation between individuals which may be effective on their gene expression patterns. According to our knowledge, for the first time this study evaluated the expression of MT1 receptor gene in gastric adenocarcinoma tissues which consistent with our previous study but with some difference in comparisons between kind of tissue expression and difference in polymorphisms. Moreover, these results show the defending role of melatonin in the GI system.
胃癌占全球癌症病例总数的8%，导致全球癌症死亡总数的10%。吲哚胺N-乙酰-5-甲氧基色胺，即褪黑素，是松果体产生的主要激素。最近，褪黑素在一些恶性肿瘤如乳腺癌和结肠癌中的抗癌作用以及在胃肠道中的一些保护作用已得到充分记录，这些作用包括自由基清除、抗有丝分裂和凋亡特性。根据褪黑素的抗癌作用、这种神经激素在胃肠道中的广泛分布以及针对这种神经激素提出的一些生理和药理作用，并且基于我们之前显示MT2受体在胃腺癌中表达的研究，本研究最初计划确定褪黑素受体MT1在腺癌患者组织样本中的表达。通过实时PCR对10名胃腺癌患者和10名正常个体进行MT1基因表达检测。此外，为了筛查我们样本中MT1的不同等位基因，开发了SSCP-PCR程序。我们的结果有趣地显示，与正常组相比，癌症组和癌旁组中MT1受体的表达很高。我们的研究结果还表明，50岁以上个体中MT1受体mRNA水平与肿瘤分级之间存在显著关联。PCR-SSCP分析结果显示个体之间存在差异，这可能对其基因表达模式产生影响。据我们所知，本研究首次评估了胃腺癌组织中MT1受体基因的表达，这与我们之前的研究一致，但在组织表达类型和多态性差异的比较上存在一些不同。此外，这些结果显示了褪黑素在胃肠道系统中的保护作用。
