Laboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics and Obesity Group) , University of the Balearic Islands , 07122 Palma , Spain.
Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center , University of the Basque Country (UPV/EHU) , Vitoria , Spain.
J Agric Food Chem. 2018 Sep 19;66(37):9679-9689. doi: 10.1021/acs.jafc.8b02611. Epub 2018 Sep 5.
We explored the potential of hesperidin and capsaicin, separately and in combination, to induce white adipose tissue (WAT) browning and to help body weight management in Western diet-fed rats. Adult male Wistar rats were fed for 8 weeks with Western diet and treated daily with hesperidin (100 mg/kg/day), capsaicin (4 mg/kg/day), hesperidin (100 mg/kg/day) + capsaicin (4 mg/kg/day), or the vehicle. Hesperidin and capsaicin separately, but not (or to a lesser extent) the combination, resulted in a decreased size of adipocytes and induced emergence of multilocular brown-like adipocytes positive for UCP1 and CIDEA in retroperitoneal WAT. Expression levels of browning markers, such as Prdm16, in inguinal WAT also increased with capsaicin treatment compared with the vehicle (145% ± 17% vs 92% ± 21%, P < 0.05), but no significant effects were found with the combination (106% ± 12%). Thus, the combination of both bioactives reduces the effectiveness of each compound to decrease the adipocyte size and induce WAT browning.
我们分别探讨了橙皮苷和辣椒素单独及联合应用，对诱导白色脂肪组织（WAT）褐变和帮助控制西式饮食喂养大鼠体重的潜力。成年雄性 Wistar 大鼠用西式饮食喂养 8 周，并用橙皮苷（100mg/kg/天）、辣椒素（4mg/kg/天）、橙皮苷（100mg/kg/天）+辣椒素（4mg/kg/天）或载体每日处理。橙皮苷和辣椒素单独应用，而不是（或在较小程度上）联合应用，可减小脂肪细胞的大小，并诱导腹膜后 WAT 中出现多房棕色样的 UCP1 和 CIDEA 阳性脂肪细胞。与载体相比，辣椒素处理也增加了腹股沟 WAT 中褐色标记物如 Prdm16 的表达水平（145%±17%比 92%±21%，P<0.05），但联合应用没有发现显著影响（106%±12%）。因此，两种生物活性物质的联合应用降低了每种化合物减少脂肪细胞大小和诱导 WAT 褐色化的效果。
