Oliveira Bmb, Ubaldini Alm, Baesso M L, Andrade Lhc, Lima S M, Giannini M, Hernandes L, Pascotto R C
Oper Dent. 2018 Sep/Oct;43(5):E253-E265. doi: 10.2341/17-366-L.
To characterize the chemical interactions and analyze the interface of adhesive systems containing 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) and N-methacryloyl glycine (methacrylamide) functional monomers with the dentin in noncarious cervical lesions (NCCLs) compared with artificial defects (ADs).
Twenty human teeth with natural NCCLs on the buccal surface were used. Class V cavities, similar to NCCLs, were created on the lingual surface to serve as controls. Teeth were randomly allocated to two groups according to the functional monomer in the adhesive (N=10): G1, 10-MDP; and G2, methacrylamide. NCCLs and ADs were characterized by their mineral composition (MC) and degree of demineralization (DD) using micro-Raman spectroscopy, adhesive/dentin chemical interactions (CIs) were assessed with infrared photoacoustic spectroscopy, and interface morphology was evaluated with scanning electron and light microscopy. MC, CI, and DD data were submitted to Shapiro-Wilk and Student t-tests ( p<0.05).
Compared with ADs, dentin in NCCLs was hypermineralized ( p<0.05). In G1, CI, and DD in the first 2 μm, and adhesive projections in NCCLs and ADs interfaces were similar. Additionally, a thin layer of dentin collagen was observed in ADs, while it was hardly present in NCCLs. In G2, although CI could not be identified, changes in the mineral components were observed. The DD in the ADs and NCCLs were statistically similar, while SEM showed a lack of adhesion at NCCLs interface. DD and collagen exposure in the ADs and NCCLs were more pronounced than in G1.
Results suggest that the G1 adhesive could be applied directly on the superficial sclerotic layer in NCCLs. In contrast, previous cavity preparation should be conducted to improve the micromechanical interaction of G2 with the dentin.
与人工缺损（ADs）相比，表征含10-甲基丙烯酰氧基癸基磷酸二氢酯（10-MDP）和N-甲基丙烯酰甘氨酸（甲基丙烯酰胺）功能单体的粘结系统与非龋性颈部病变（NCCLs）中牙本质的化学相互作用并分析其界面。
使用20颗颊面有天然NCCLs的人牙。在舌面制备类似于NCCLs的V类洞作为对照。根据粘结剂中的功能单体将牙齿随机分为两组（N = 10）：G1组，含10-MDP；G2组，含甲基丙烯酰胺。使用显微拉曼光谱法通过矿物组成（MC）和脱矿程度（DD）对NCCLs和ADs进行表征；用红外光声光谱法评估粘结剂/牙本质化学相互作用（CIs）；用扫描电子显微镜和光学显微镜评估界面形态。将MC、CI和DD数据进行Shapiro-Wilk检验和Student t检验（p < 0.05）。
与ADs相比，NCCLs中的牙本质矿化过度（p < 0.05）。在G1组中，前2μm内的CI和DD以及NCCLs和ADs界面中的粘结剂突起相似。此外，在ADs中观察到一层薄的牙本质胶原，而在NCCLs中几乎不存在。在G2组中，虽然无法识别CI，但观察到矿物成分的变化。ADs和NCCLs中的DD在统计学上相似，而扫描电子显微镜显示NCCLs界面缺乏粘结。ADs和NCCLs中的DD和胶原暴露比G1组更明显。
结果表明，G1粘结剂可直接应用于NCCLs的浅表硬化层。相比之下，应先进行窝洞预备以改善G2与牙本质的微机械相互作用。
