Department of Dermatology, the Renmin Hospital of Tongchuan City, Shanxi, China.
Department of Clinical Laboratory, The 2nd Affiliated Hospital of Guizhou Medical College, Guizhou, China.
Clin Exp Rheumatol. 2019 Mar-Apr;37(2):242-253. Epub 2018 Aug 29.
Interleukin-10 (IL-10) polymorphisms have been reported to be associated with systemic lupus erythematosus (SLE), however, the results are controversial. Therefore, we conducted a meta-analysis with trial sequential analysis to evaluate a more accurate estimation of the associations.
Eligible studies were retrieved by searching PubMed, Embase, Google Scholar, VIP, Wan Fang and China National Knowledge Infrastructure databases. Hardy-Weinberg equilibrium (HWE) was evaluated. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. Heterogeneity was evaluated by Q statistic and I2 statistic. Sensitivity analysis and subgroup analysis (stratified by HWE, region, event sample size, source of controls, genotyping method) were conducted and the potential for publication bias was assessed. Trial sequential analysis was introduced to assess the information size and the positive results.
Twenty case-control studies were included. Overall results from IL10-1082A/G polymorphism showed increased risk of systemic lupus erythematosus, but no significant associations were observed in both IL10-819C/T and IL10-592C/A polymorphism. Increased risk of SLE was also observed in IL10A/G polymorphism in Asian population, hospital-based and PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism) subgroups. In addition, decreased risk of SLE was widely detected in IL10-819C/T and IL10-592C/A polymorphisms in subgroup analysis.
Our study suggests that the IL10-1082A/G polymorphism is a risk factor in systemic lupus erythematosus. A decreased risk of SLE in the IL10-819C/T and IL10-592C/A polymorphisms in subgroups was also observed, but further rigorously studies are needed to confirm these results.
白细胞介素-10（IL-10）多态性与系统性红斑狼疮（SLE）有关，但结果存在争议。因此，我们进行了一项荟萃分析和试验序贯分析，以评估更准确的关联估计。
通过检索 PubMed、Embase、Google Scholar、VIP、万方和中国知网数据库，检索到符合条件的研究。评估 Hardy-Weinberg 平衡（HWE）。计算比值比（ORs）和 95%置信区间（CIs）。采用 Q 统计量和 I2 统计量评估异质性。进行敏感性分析和亚组分析（按 HWE、地区、事件样本量、对照来源、基因分型方法分层），并评估潜在的发表偏倚。引入试验序贯分析评估信息大小和阳性结果。
共纳入 20 项病例对照研究。总体结果表明，IL10-1082A/G 多态性增加了系统性红斑狼疮的风险，但在 IL10-819C/T 和 IL10-592C/A 多态性中没有观察到显著关联。在亚洲人群、医院为基础和 PCR-RFLP（聚合酶链反应限制性片段长度多态性）亚组中，IL10A/G 多态性也观察到 SLE 风险增加。此外，在亚组分析中，IL10-819C/T 和 IL10-592C/A 多态性广泛检测到 SLE 风险降低。
我们的研究表明，IL10-1082A/G 多态性是系统性红斑狼疮的一个危险因素。在亚组分析中，IL10-819C/T 和 IL10-592C/A 多态性也观察到 SLE 风险降低，但需要进一步严格的研究来证实这些结果。
