CSIR - Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
CSIR - Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:297-306. doi: 10.1016/j.msec.2018.06.050. Epub 2018 Jun 25.
Development of biomaterials for tissue engineering applications is of great interest to meet the demand of different clinical requirements. The wound heal dressing biomaterials should necessarily contain well-defined therapeutic components and desirable physical, chemical and biological properties to support optimal delivery of therapeutics at the site of the wound. In this study, we developed collagen-silica wound heal scaffold incorporated with the extract of Cynodon dactylon, characterized and evaluated for its wound heal potential in vitro and in vivo against collagen (Col) and Collagen-silica (CS) scaffolds that served as controls. The prepared Collagen-Silica-Cynodon extract (CSCE) scaffold exhibits porous morphology with preferable biophysical, chemical, mechanical and mass transfer properties besides its controlled biodegradation at the wound site. Stability of CSCE was found to be better than that of native collagen due to intermolecular interactions between collagen and constituents of C. dactylon as confirmed by FTIR analysis. Notably, in vitro biocompatibility assay using DAPI and Rhodamine 123 staining demonstrated that the proliferation of NIH3T3 fibroblast cells was better for CSCE when compared to the Col and CS scaffolds. In vivo wound healing experiments with full-thickness excision wounds in wistar rat model demonstrated that the wounds treated with CSCE showed accelerated healing with enhanced collagen deposition when compared to wounds treated with Col and CS scaffolds, and these studies substantiated the efficacy of CSCE scaffold for treating wounds.
用于组织工程应用的生物材料的开发对于满足不同临床需求具有重要意义。伤口愈合敷料生物材料必须含有明确的治疗成分和理想的物理、化学和生物学特性，以支持在伤口部位最佳输送治疗剂。在这项研究中，我们开发了一种胶原蛋白-二氧化硅伤口愈合支架，其中掺入了狗牙根的提取物，对其在体外和体内的伤口愈合潜力进行了表征和评估，与作为对照的胶原蛋白（Col）和胶原蛋白-二氧化硅（CS）支架进行了比较。所制备的胶原蛋白-二氧化硅-狗牙根提取物（CSCE）支架具有多孔形态，具有较好的物理、化学、机械和传质性能，并且在伤口部位具有可控的生物降解性。CSCE 的稳定性由于胶原蛋白与狗牙根成分之间的分子间相互作用，优于天然胶原蛋白，这一点通过傅里叶变换红外（FTIR）分析得到了证实。值得注意的是，使用 DAPI 和罗丹明 123 染色进行的体外细胞相容性试验表明，与 Col 和 CS 支架相比，CSCE 更有利于 NIH3T3 成纤维细胞的增殖。在 wistar 大鼠全层切除伤口模型中的体内伤口愈合实验表明，与 Col 和 CS 支架处理的伤口相比，用 CSCE 处理的伤口显示出更快的愈合速度，胶原蛋白沉积增加，这些研究证实了 CSCE 支架治疗伤口的功效。
