Polymer Materials Research Center, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China.
Polymer Materials Research Center, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:338-348. doi: 10.1016/j.msec.2018.06.062. Epub 2018 Jul 2.
In this study, novel molecularly imprinted polymer (MIP) microparticles containing methacryl polyhedral oligomeric silsesquioxane (M-POSS) were synthesized through the RAFT precipitation polymerization (RAFTPP) method using paclitaxel (PTX) as the templates. During the course of this investigation, methacrylic acid (MAA) was used as the functional monomer, while ethylene glycol dimethacrylate (EGDMA) was utilized as a cross-linker. The effects of different molar ratios of M-POSS on the microparticles were characterized. The obtained MIP microparticles were confirmed by FT-IR and TGA-DSC. The results of SEM showed regular spherical-shaped MIP microparticles with diameters around 170-490 nm. The PTX loading quantity was closely correlated with the content of M-POSS, and the MIP microparticles showed a satisfactory affinity to PTX with high drug loading (17.1%) and encapsulation efficiency (85.5%). Moreover, these MIP microparticles were sensitive to pH, and consequently the release rates of PTX at pH 5 were much faster than those at pH 7 due to the acid cleavage of the hydrogen bonds. In addition, the results from release experiments of the MIP microparticles showed a very slow and controlled release of PTX, which heralded promising potential as a carrier for PTX delivery in cancer therapy.
在这项研究中，通过 RAFT 沉淀聚合（RAFTPP）方法，使用紫杉醇（PTX）作为模板，合成了新型的含有甲丙烯酸多面体低聚倍半硅氧烷（M-POSS）的分子印迹聚合物（MIP）微球。在研究过程中，使用甲基丙烯酸（MAA）作为功能单体，而乙二醇二甲基丙烯酸酯（EGDMA）则用作交联剂。研究了不同摩尔比的 M-POSS 对微球的影响。通过傅里叶变换红外光谱（FT-IR）和热重分析-差示扫描量热法（TGA-DSC）对获得的 MIP 微球进行了确认。SEM 结果表明，MIP 微球具有规则的球形，直径约为 170-490nm。PTX 的负载量与 M-POSS 的含量密切相关，MIP 微球对 PTX 具有较高的药物负载量（17.1%）和包封效率（85.5%），表现出良好的亲和力。此外，这些 MIP 微球对 pH 值敏感，因此在 pH 5 时，由于氢键的酸裂解，PTX 的释放速率比在 pH 7 时快得多。此外，MIP 微球的释放实验结果表明，PTX 的释放非常缓慢且可控，这预示着其在癌症治疗中作为 PTX 传递载体具有很大的潜力。
