Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:726-736. doi: 10.1016/j.msec.2018.07.014. Epub 2018 Jul 4.
In this study, a poly l-lactic acid (PLLA) fibrous mat was prepared by electrospinning, followed by surface modification with polydopamine (PDA), based on its strong adhesion performance and self-polymerization of dopamine. The PDA coating on the fibrous mat surface provided a reaction platform for heparin via a Michael-type addition reaction and a reductive surface for Ag in situ formation of silver nanoparticles (AgNPs) in AgNO solution. The structure and chemical composition of the fibrous mats were determined by scanning electronic microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. All the results confirmed the successful immobilization of heparin and AgNPs on the PLLA fibrous mats. Thermogravimetric analysis (TGA) and energy dispersive X-ray spectrum (EDS) analysis were used to determine the content of AgNPs and their distribution on the fibrous mat surface. Water contact angle measurements showed the hydrophilic improvement after modification. The antibacterial investigation indicated that the fibrous mats could inhibit the growth of both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Protein adsorption, the hemolysis test, the coagulation test, complement activation, and platelet activation were used to confirm the compatibility with blood and the anticoagulation property of the fibrous mats. Finally, cell proliferation and live/dead assays, conducted with cultured fibroblasts on the fibrous mats, showed that the modified fibrous mat surface had good cell compatibility. This antibacterial/anticoagulant dual-functional surface, based on poly l-lactic acid electrospun fibrous mats, would have potential application in blood contacting materials.
在这项研究中，通过静电纺丝制备了聚 L-乳酸（PLLA）纤维垫，然后通过多巴胺的强粘附性能和自聚合对其进行了聚多巴胺（PDA）表面改性。纤维垫表面的 PDA 涂层为肝素提供了一个迈克尔型加成反应的反应平台，并为 AgNO 溶液中银纳米粒子（AgNPs）的原位还原表面提供了一个反应平台。扫描电子显微镜（SEM）、X 射线光电子能谱（XPS）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）对纤维垫的结构和化学组成进行了测定。所有结果均证实肝素和 AgNPs成功固定在 PLLA 纤维垫上。热重分析（TGA）和能谱分析（EDS）分析用于确定 AgNPs 的含量及其在纤维垫表面的分布。水接触角测量表明修饰后亲水性提高。抗菌研究表明，纤维垫可以抑制大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的生长。蛋白质吸附、溶血试验、凝血试验、补体激活和血小板激活用于证实纤维垫与血液的相容性和抗凝性能。最后，通过纤维垫上培养的成纤维细胞进行细胞增殖和死活试验，表明改性纤维垫表面具有良好的细胞相容性。这种基于聚 L-乳酸静电纺丝纤维垫的抗菌/抗凝双重功能表面在与血液接触的材料中具有潜在的应用前景。
