Peng Zhao, Shibata Nao, Tada Hideaki, Kaneko Jun
a Department of Microbial Biotechnology , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
b Exploratory Research Laboratories , Tsukuba Research Institute, ONO pharmaceutical Co., LTD , Tsukuba , Japan.
Biosci Biotechnol Biochem. 2018 Dec;82(12):2094-2097. doi: 10.1080/09168451.2018.1515614. Epub 2018 Sep 5.
CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.
使用表达人CCR5的CHO细胞研究了葡萄球菌双组分毒素的CCR5介导的细胞毒性。LukE和Hlg2之间边缘结构域环交换突变体的细胞毒性表明，LukE的环4与LukD结合时对细胞毒性至关重要。有趣的是，Hlg2表现出LukF依赖性的CCR5介导的细胞毒性，这表明毒素的F组分在细胞特异性细胞毒性中也起作用。
