Howley P M, Schenborn E T, Lund E, Byrne J C, Dahlberg J E
Mol Cell Biol. 1985 Nov;5(11):3310-5. doi: 10.1128/mcb.5.11.3310-3315.1985.
We constructed a mutant of bovine papillomavirus type 1 (BPV-1) DNA that lacked a transcriptional enhancer located 3' to the polyadenylation site of the early viral RNAs expressed in transformed cells. This mutant DNA, when separated from the procaryotic sequences, transforms mouse cells with an efficiency comparable to that of the full BPV-1 genome, and it exists as a stable multicopy plasmid in transformed cells. The BPV-1 distal enhancer suppresses the effects of a cis-inhibitory element in pML2 sequences but is not essential for the expression of the viral genes involved in cellular transformation or plasmid maintenance.
我们构建了1型牛乳头瘤病毒（BPV-1）DNA的一个突变体，该突变体缺失位于转化细胞中表达的早期病毒RNA聚腺苷酸化位点3'端的转录增强子。当该突变体DNA与原核序列分离后，其转化小鼠细胞的效率与完整的BPV-1基因组相当，并且在转化细胞中以稳定的多拷贝质粒形式存在。BPV-1远端增强子可抑制pML2序列中顺式抑制元件的作用，但对于参与细胞转化或质粒维持的病毒基因的表达并非必需。
