Böhning Dankmar, Böhning Walailuck, Guha Nishan, Cowan David A, Bartlett Christiaan, Sönksen Peter H, Holt Richard I G
Southampton Statistical Sciences Research Institute, University of Southampton, Southampton, SO17 1BJ, UK.
Human Development and Health Academic Unit, Faculty of Medicine, Southampton General Hospital, University of Southampton, Southampton, SO16 6YD, UK.
BMC Res Notes. 2018 Sep 5;11(1):650. doi: 10.1186/s13104-018-3741-7.
The GH-2000 biomarker test has been introduced by the World Anti-Doping Agency as a method of detecting growth hormone misuse in professional sport. The test involves the measurement insulin-like growth factor-I and the amino-terminal pro-peptide of type III collagen (P-III-NP) which increase in a dose-dependent manner in response to GH. These measurements are combined in sex specific formulae that include an age adjustment. The original age adjustment overcorrects the effect of age in male athletes and could potentially place older men at a disadvantage. The purpose of this note is to investigate the performance of a previously suggested correction term in two new and larger data sets.
The GH-2000 score was calculated for 7307 samples obtained from 15 accredited WADA laboratories in 2017 and 3916 samples measured at Drug Control Centre, King's College London, UK between 2013 and 2017. The GH-2000 scores were investigated for positive age effects using standard regression modelling. As previously, all analyses confirmed a positive age effect. Applying the earlier suggested correction term of 0.032 × age showed a significant over-correction leading to a negative association of the GH-2000 score with age. We now suggest a smaller age correction of 0.020 × age, which corresponds to the smallest effect found in the earlier studies.
世界反兴奋剂机构引入了GH - 2000生物标志物检测方法，用于检测职业体育中生长激素的滥用情况。该检测涉及测量胰岛素样生长因子 - I和III型胶原蛋白的氨基末端前肽（P - III - NP），它们会随着生长激素的作用呈剂量依赖性增加。这些测量值被纳入包含年龄校正的性别特异性公式中。最初的年龄校正对男性运动员的年龄影响校正过度，可能会使年长男性处于不利地位。本报告的目的是在两个新的更大数据集中研究先前提出的校正项的性能。
计算了2017年从15个获得世界反兴奋剂机构认可的实验室获取的7307个样本以及2013年至2017年期间在英国伦敦国王学院药物控制中心测量的3916个样本的GH - 2000分数。使用标准回归模型研究了GH - 2000分数的正年龄效应。与之前一样，所有分析均证实了正年龄效应。应用先前建议的0.032×年龄的校正项显示出显著的过度校正，导致GH - 2000分数与年龄呈负相关。我们现在建议使用较小的年龄校正值0.020×年龄，这与早期研究中发现的最小效应相对应。
