Debbarma Debajit, Uppal Varinder, Bansal Neelam, Gupta Anuradha
Department of Veterinary Anatomy, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India.
Dermatol Res Pract. 2018 Aug 16;2018:5345390. doi: 10.1155/2018/5345390. eCollection 2018.
The study was conducted on skin of 24 buffaloes collected from slaughter house. The skin tissues were collected from dorsal, lateral, and ventral parts of head, neck, thorax, abdomen, and tail regions and fixed in 10% neutral buffered formalin. The tissues were processed for paraffin blocks preparation by acetone benzene schedule. The paraffin sections of 5-6 m were cut with rotary microtome and stained with hematoxylin and eosin. The sweat glands in buffaloes were of saccular and simple coiled tubular type. Most of the sweat glands were associated with hair follicles and consisted of a coiled secretory portion (body) and a straight duct. The secretory portion was made up of glandular tubules, myoepithelium, and basement membrane. The duct portion had a narrow lumen and was lined by simple cuboidal epithelium. The glandular epithelium was simple squamous, simple cuboidal, or low columnar type depending upon their stage of secretary activity. Two types of sweat glands were observed, i.e., apocrine and merocrine. Large number of blood vessels and nerve fibers were observed in the vicinity of the sweat glands. In head, neck, and tail regions the maximum number of sweat glands/mm was observed in dorsal side which did not vary significantly (p<0.05) from lateral and ventral side. In abdomen region the number of sweat glands/mm was maximum on lateral region which varied significantly from ventral region (p<0.05). Overall, the maximum number of sweat glands/mm was in head region followed by abdomen, thorax, neck, and tail but without any significant (p<0.05) difference. Maximum sweat gland diameter was found in abdomen region followed by thorax, head, neck, and tail region.
该研究在从屠宰场收集的24头水牛的皮肤上进行。皮肤组织取自头部、颈部、胸部、腹部和尾部区域的背侧、外侧和腹侧部分，并固定于10%中性缓冲福尔马林中。组织通过丙酮 - 苯程序处理以制备石蜡块。用旋转切片机切出5 - 6μm的石蜡切片，并用苏木精和伊红染色。水牛的汗腺为囊状和单盘曲管状类型。大多数汗腺与毛囊相关，由盘曲的分泌部（主体）和直导管组成。分泌部由腺小管、肌上皮和基底膜组成。导管部分管腔狭窄，内衬单层立方上皮。腺上皮根据其分泌活动阶段为单层扁平、单层立方或低柱状类型。观察到两种类型的汗腺，即顶泌汗腺和外泌汗腺。在汗腺附近观察到大量血管和神经纤维。在头部、颈部和尾部区域，背侧每平方毫米的汗腺数量最多，与外侧和腹侧相比无显著差异（p<0.05）。在腹部区域，外侧每平方毫米的汗腺数量最多，与腹侧区域有显著差异（p<0.05）。总体而言，每平方毫米汗腺数量最多的是头部区域，其次是腹部、胸部、颈部和尾部，但无显著（p<0.05）差异。汗腺最大直径出现在腹部区域，其次是胸部、头部、颈部和尾部区域。
