Department of Clinical and Experimental Medicine, Messina University, Italy.
Department of Biomedical and Dental Sciences and Morphofunctional Imaging, Messina University, Italy.
Biomed Res Int. 2018 Aug 13;2018:9853765. doi: 10.1155/2018/9853765. eCollection 2018.
The use of equine bone blocks is widely reported for bone augmentation techniques. The block must be shaped according to the form of the defect that should be regenerated. The shaping could be performed by hand before or during the surgery, in a sterile ambient, or using a CNC milling machine that could not be sterile. The aim of our study was to evaluate if a steam sterilization could provide a medical grade sterilization of the blocks and to evaluate if bone microstructure and collagen structures change after different steam sterilization protocols provided by mainstream autoclave.
Two blocks of equine bone were divided into 16 samples. 1 sample was used as control and not submitted to any treatment. 15 samples were infected with a Streptococcus faecalis bacterial culture. The samples were singularly packed, randomly divided into 3 groups, and submitted to autoclave sterilization on the same device. The groups were submitted to a sterilization cycle (Gr. A: 121°C, 1,16 bar for 20'; Gr. B:134°C, 2,16 bar for 4'; Gr. C: 134°C, 2,16 bar for 3.30 min.). 2 samples for each group were evaluated for the sterility. 3 samples for each group were observed at SEM to notice the macro- and microstructure modification and to confocal microscope to observe the collagen.
All samples were sterile. The SEM evaluation showed, in all groups, a preserved morphological structure. Confocal microscope evaluation shows that the collagen structure appears to be more uniform and preserved in group C.
Data show that autoclave steam sterilization could be reliable to obtain sterilization of equine bone blocks.
马骨块被广泛应用于骨增量技术。块必须根据要再生的缺陷形状进行塑形。该塑形可以在手术前或手术中，在无菌环境下，或使用不能无菌的 CNC 铣床进行。我们的研究目的是评估蒸汽灭菌是否可以为骨块提供医疗级别的灭菌，并评估主流灭菌器提供的不同蒸汽灭菌方案是否会改变骨的微观结构和胶原结构。
将两块马骨分为 16 个样本。1 个样本作为对照，不进行任何处理。15 个样本被感染粪肠球菌细菌培养物。样本单独包装，随机分为 3 组，在同一设备上进行高压灭菌器灭菌。将这些组分别进行灭菌循环（Gr. A：121°C，1.16 巴 20'；Gr. B：134°C，2.16 巴 4'；Gr. C：134°C，2.16 巴 3.30 分钟）。每组 2 个样本用于评估无菌性。每组 3 个样本用于 SEM 观察宏观和微观结构的变化，以及共聚焦显微镜观察胶原。
所有样本均无菌。SEM 评估显示，在所有组中，形态结构均得到保留。共聚焦显微镜评估显示，胶原结构在组 C 中似乎更均匀和保留。
数据表明，高压蒸汽灭菌可以可靠地获得马骨块的灭菌。
