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在缅甸山羊中首次检测到鲁氏泰勒虫的分子证据

First molecular detection of Theileria luwenshuni from goats in Myanmar.

作者信息

Bawm Saw, Kakisaka Keita, Thu May June, Chel Hla Myet, Oo Yamon Min Nyunt, Soe Nyein Chan, Win Shwe Yee, Htun Lat Lat, Win Mar Mar, Suzuki Hitoshi, Nakao Ryo, Katakura Ken

机构信息

Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.

Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar.

出版信息

Parasitol Res. 2018 Oct;117(10):3361-3364. doi: 10.1007/s00436-018-6073-6. Epub 2018 Sep 5.

Abstract

Tick-borne intracellular protozoan parasites of the Theileria genus infect a wide range of both domestic and wild animals. In the present study, we describe the first PCR detection of Theileria luwenshuni in the blood of goats in Myanmar. Nested PCR targeting the Theileria 18S rRNA gene resulted in seven positive goats in central and northern Myanmar. Nucleotide sequencing of the PCR products revealed that all seven sequences were identical and showed 100% identity with T. luwenshuni sequences in GenBank from goats and sheep in China. Since T. luwenshuni parasites have recently been discovered and shown to have nationwide distribution in China, they might have been introduced into Myanmar via transboundary movement of infected domestic small ruminants and/or wild animals from China.

摘要

泰勒虫属的蜱传细胞内原生动物寄生虫感染多种家畜和野生动物。在本研究中,我们描述了在缅甸山羊血液中首次通过聚合酶链反应(PCR)检测到吕氏泰勒虫。针对泰勒虫18S核糖体RNA(rRNA)基因的巢式PCR在缅甸中部和北部检测到7只阳性山羊。PCR产物的核苷酸测序显示,所有7个序列均相同,并且与来自中国山羊和绵羊的GenBank中吕氏泰勒虫序列具有100%的同一性。由于吕氏泰勒虫寄生虫最近在中国被发现并显示在全国范围内分布,它们可能是通过受感染的家养小反刍动物和/或来自中国的野生动物的跨境移动而传入缅甸的。

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