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菲律宾山羊中 、 和 的首次分子鉴定

First Molecular Identification of , , and in Goats from the Philippines.

作者信息

Galon Eloiza May, Ybañez Rochelle Haidee, Macalanda Adrian Miki, Estabillo Giemelene Rose, Montano Margaret Therese Rose, Veedor Marielle Danise, Garvida Anatolio, Fabon Ralph Joselle, Callanta Mary Ruth, Labutong Kim Joseph, Tumwebaze Maria Agnes, Byamukama Benedicto, Ji Shengwei, Zafar Iqra, Ybañez Adrian, Xuan Xuenan

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.

Institute of Molecular Parasitology and Protozoan Diseases, Main Campus and College of Veterinary Medicine, Barili Campus, Cebu Technological University, Cebu City 6000, Philippines.

出版信息

Pathogens. 2022 Sep 27;11(10):1109. doi: 10.3390/pathogens11101109.

Abstract

Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused by apicomplexan parasites spp. and spp., while anaplasmosis is caused by bacterial spp. In the Philippines, the presence of , , and has not been reported in goats. In this study, DNA obtained from goats were molecularly screened for and . Of 396, 77.02% (305/396) and 38.64% (153/396) were positive for piroplasma and using PCR assays targeting the 18S rRNA and 16S rRNA genes, respectively. Similarly, was detected in six samples (1.52%). Representative sequences shared 89.97-97.74% identity with each other and were most closely related to , , and spp. Meanwhile, 16SrRNA sequences were related to , and . This is the first molecular identification of spp., and spp. in goats from the Philippines.

摘要

山羊是重要的家畜,养羊是小农户的创收项目,对世界许多地区的农业发展起到了支撑作用。然而,山羊生产常常受到多种因素的限制,比如蜱传疾病。山羊梨形虫病是一种由顶复门寄生虫属和属引起的疾病,而无浆体病则由细菌属引起。在菲律宾,尚未有关于山羊感染、和的报道。在本研究中,对从山羊身上获取的DNA进行了和的分子筛查。在396份样本中,分别使用针对18S rRNA和16S rRNA基因的PCR检测方法,发现77.02%(305/396)的样本梨形虫呈阳性,38.64%(153/396)的样本呈阳性。同样,在6份样本(1.52%)中检测到了。代表性的序列彼此之间的同源性为89.97 - 97.74%,与、和属的关系最为密切。同时,16S rRNA序列与、和相关。这是菲律宾山羊中首次对属和属进行分子鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c61f/9612162/dac3c656906c/pathogens-11-01109-g001.jpg

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