Han Yuefeng, Chen Deshang, Li Hui, Wang Xiaomin, Zhang Mingjie, Yang Yang
Department of Otolaryngology, Head and Neck surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Jul 30;38(8):923-930. doi: 10.3969/j.issn.1673-4254.2018.08.04.
To investigate the effect of knocking down long chain non-coding RNA MALAT-1 gene on the biologicalbehaviors of human laryngeal squamous cell carcinoma Hep-2 cells.
With immortalized nasopharyngeal epithelial(NPE) cell line NP-69 as the reference, MALAT1 expression in FaDu, Hep-2 and nasopharyngeal carcinoma CNE-2Z cells weredetected using real-time PCR. Hep-2 cells were transfected with shmalat1 lentivirus and the expression of MALAT1 wasdetected. MTT assay, flow cytometry, Transwell assay and M Atrigel invasiveness test were used to evaluate the effect ofMALAT-1 knockdown on the proliferation, cell cycle, cell apoptosis, migration, and invasiveness of Hep-2 cells.
Compared with NP-69 cells, Hep-2 cells, FaDu cells, and CNE-2Z cells all showed significantly increased MALAT-1expression. In Hep-2 cells, knockdown of MALAT-1 significantly inhibited the cell proliferation, increased the cell percentagein S phase ( < 0.01), decreased the cell percentage in G2/M phase ( < 0.01), and attenuated the migration and invasiveness of thecells.
MALAT-1 is over-expressed in laryngeal squamous cell carcinoma, and knocking down MALAT-1 gene cansignificantly suppress the proliferation, invasion and migration and promotes apoptosis of the cancer cells.
探讨敲低长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞生物学行为的影响。
以永生化鼻咽上皮（NPE）细胞系NP-69为参照，采用实时荧光定量PCR检测FaDu、Hep-2和鼻咽癌CNE-2Z细胞中MALAT1的表达。用shmalat1慢病毒转染Hep-2细胞，检测MALAT1的表达。采用MTT法、流式细胞术、Transwell法和基质胶侵袭试验评估敲低MALAT-1对Hep-2细胞增殖、细胞周期、细胞凋亡、迁移和侵袭能力的影响。
与NP-69细胞相比，Hep-2细胞、FaDu细胞和CNE-2Z细胞中MALAT-1表达均显著升高。在Hep-2细胞中，敲低MALAT-1可显著抑制细胞增殖，增加S期细胞百分比（<0.01），降低G2/M期细胞百分比（<0.01），并减弱细胞的迁移和侵袭能力。
MALAT-1在喉鳞状细胞癌中过表达，敲低MALAT-1基因可显著抑制癌细胞的增殖、侵袭和迁移，并促进其凋亡。
