School of Kinesiology, Auburn University, Auburn, Alabama (Dr Peters, Dr Ballmann, Ms Zehner, Mr McCroskey); Department of Health and Human Performance (Ms Quindry, Dr Dumke, Dr Quindry); Center for Environmental Health Sciences (Mr Ferguson); School of Public and Community Health Sciences (Dr Ward), University of Montana, Missoula, Montana.
J Occup Environ Med. 2018 Dec;60(12):1073-1081. doi: 10.1097/JOM.0000000000001437.
The current laboratory study quantified blood oxidative stress to woodsmoke exposure.
Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 μg/m], Low Exposure [250 μg/m], and High Exposure [500 μg/m], Woodsmoke [particulate matter less than 2.5 μm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples.
UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air.
Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.
本实验室研究定量分析了血氧化应激与木柴燃烧烟雾暴露的关系。
参与者在三个随机交叉运动试验（清洁空气[0μg/m]、低暴露[250μg/m]和高暴露[500μg/m]、木柴燃烧烟雾[小于 2.5μm 的颗粒物，PM2.5]）中吸入木柴燃烧烟雾。在血液样本采集前（Pre）、吸入后即刻（Immediately Post）和 1 小时（1Hr）后，分别检测 Trolox 等效抗氧化能力（TEAC）、尿酸（UA）、8-异前列腺素（8-ISO）、脂质过氧化物（LOOH）、蛋白羰基（PC）、硝基酪氨酸（3-NT）、8-异前列腺素和髓过氧化物酶（MPO）的水平。
低暴露组 UA 在吸入后即刻下降，而 TEAC 水平在吸入后和 1 小时后增加。高暴露组 1 小时后 LOOH 水平下降，而 8-ISO 在两次烟雾暴露后均增加。PC 和 MPO 在所有试验后均无变化，而 3-NT 在清洁空气组后增加。
血液氧化应激的发生在很大程度上与 PM2.5 浓度无关。未来的研究应采用更长时间的烟雾暴露和运动，并结合生理参数进行研究。
