野地消防员模拟训练后炎症和氧化应激的肺部及全身测量指标
Measured Pulmonary and Systemic Markers of Inflammation and Oxidative Stress Following Wildland Firefighter Simulations.
作者信息
Ferguson Matthew D, Semmens Erin O, Dumke Charles, Quindry John C, Ward Tony J
机构信息
University of Montana, Center for Environmental Health Sciences (Dr Ferguson); University of Montana, School of Public and Community Health Sciences (Drs Semmens, Ward); University of Montana, Department of Health and Human Performance (Dr Dumke), Missoula; Auburn University, Department of Kinesiology (Dr Quindry), Alabama.
出版信息
J Occup Environ Med. 2016 Apr;58(4):407-13. doi: 10.1097/JOM.0000000000000688.
OBJECTIVE
A controlled human exposure study was conducted to investigate the impact of inhalational exposures to wood smoke PM2.5 on measured concentrations of airway and systemic inflammatory biomarkers.
METHODS
Mimicking wildland firefighter activities, 10 participants were exposed to three doses of wood smoke PM2.5 (filtered-air, 250 μg/m, and 500 μg/m) while exercising on a treadmill. Exhaled breath condensate (EBC) and blood plasma samples were obtained pre-, immediately post-, and 1-hour postexposure. 8-isoprostane, pH, and myeloperoxidase were measured in EBC, while H2O2, surfactant protein D, and pentraxin-3 (PTX3) were measured in both EBC and plasma.
RESULTS
Only pH, 8-isoprostane, and PTX3 displayed significant changes when comparing pre- and postexposures.
CONCLUSIONS
Markers of inflammation and oxidative stress, including PTX3, pH, and 8-isoprostane in EBC and/or plasma, are sensitive to wood smoke inhalation, with further investigations warranted.
目的
开展一项人体对照暴露研究，以调查吸入木烟细颗粒物（PM2.5）对气道和全身炎症生物标志物测量浓度的影响。
方法
模拟野外消防员的活动，10名参与者在跑步机上锻炼时，暴露于三剂木烟PM2.5（过滤空气、250μg/m³和500μg/m³）环境中。在暴露前、暴露后即刻和暴露后1小时采集呼出气冷凝物（EBC）和血浆样本。在EBC中测量8-异前列腺素、pH值和髓过氧化物酶，而在EBC和血浆中均测量过氧化氢、表面活性蛋白D和五聚素-3（PTX3）。
结果
比较暴露前后，只有pH值、8-异前列腺素和PTX3出现了显著变化。
结论
EBC和/或血浆中的炎症和氧化应激标志物，包括PTX3、pH值和8-异前列腺素，对吸入木烟敏感，有必要进一步开展研究。
Zotero 插件