Research Center for Applied Science, Academia Sinica, Taiwan.
Analyst. 2018 Sep 24;143(19):4715-4722. doi: 10.1039/c8an01127c.
The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; as such, surface plasmon resonance (SPR) biosensor-based technology has become of high interest. Here, we developed an automated multiplex transmissive surface plasmon resonance (t-SPR) platform with the use of a capped gold nanoslit integrated microfluidic surface plasmon resonance (SPR) biosensor. The automated platform was custom designed to allow the analysis of spectral measurements using wavelength shift (dλ), intensity (dI) and novel area change (dA) for surface binding reactions. A simple and compact nanostructure based biosensor was fabricated with multiplex real-time detection capabilities. The sensitivity and specificity of the microfluidic device was demonstrated through the use of functionalised AuNPs for target molecule isolation and signal enhancement in combination with probes on the CG nanoslit surface. Our work allows for the multiplex detection of miRNA at femtomolar concentrations in complex media such as urine.
短链核酸生物标志物（如 miRNAs）的临床评估可能为监测疾病进展提供有用信息，并促使做出明确的治疗决策。在过去十年中，生物传感技术的进步促使人们转向快速、实时和无标记检测系统；因此，基于表面等离子体共振（SPR）生物传感器的技术引起了广泛关注。在这里，我们使用带帽金纳米狭缝集成微流控表面等离子体共振（SPR）生物传感器开发了一种自动化多路透射表面等离子体共振（t-SPR）平台。该自动化平台经过定制设计，可分析光谱测量值，包括波长位移（dλ）、强度（dI）和新的面积变化（dA），用于表面结合反应。该生物传感器具有简单紧凑的纳米结构，具有多路实时检测功能。通过使用功能化的 AuNPs 进行目标分子分离和信号增强，并结合 CG 纳米狭缝表面上的探针，证明了微流体装置的灵敏度和特异性。我们的工作允许在尿液等复杂介质中以飞摩尔浓度对 miRNA 进行多重检测。
