Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan.
Sci Rep. 2019 May 10;9(1):7204. doi: 10.1038/s41598-019-43442-w.
A variety of physiological and pathological processes rely on cell adhesion, which is most often tracked by changes in cellular morphology. We previously reported a novel gold nanoslit-based biosensor that is capable of real-time and label-free monitoring of cell morphological changes and cell viability. However, the preparation of gold biosensors is inefficient, complicated and costly. Recently, nanostructure-based aluminum (Al) sensors have been introduced for biosensing applications. The Al-based sensor has a longer decay length and is capable of analyzing large-sized mass such as cells. Here, we developed two types of double-layer Al nanoslit-based plasmonic biosensors, which were nanofabricated and used to evaluate the correlation between metastatic potency and adhesion of lung cancer and melanoma cell lines. Cell adhesion was determined by Fano resonance signals that were induced by binding of the cells to the nanoslit. The peak and dip of the Fano resonance spectrum respectively reflected long- and short-range cellular changes, allowing us to simultaneously detect and distinguish between focal adhesion and cell spreading. Also, the Al nanoslit-based biosensor chips were used to evaluate the inhibitory effects of drugs on cancer cell spreading. We are the first to report the use of double layer Al nanoslit-based biosensors for detection of cell behavior, and such devices may become powerful tools for anti-metastasis drug screening in the future.
各种生理和病理过程依赖于细胞黏附，而细胞形态的变化通常是追踪黏附的指标。我们之前报道了一种新型基于金纳米狭缝的生物传感器，该传感器能够实时、无标记地监测细胞形态变化和细胞活力。然而，金生物传感器的制备效率低、过程复杂且昂贵。最近，基于纳米结构的铝（Al）传感器已被引入用于生物传感应用。基于 Al 的传感器具有更长的衰减长度，能够分析细胞等大型质量。在这里，我们开发了两种基于双层 Al 纳米狭缝的等离子体生物传感器，它们通过纳米制造并用于评估肺癌和黑色素瘤细胞系的转移能力与黏附之间的相关性。细胞黏附通过纳米狭缝与细胞结合诱导的 Fano 共振信号来确定。Fano 共振谱的峰和谷分别反映了长程和短程细胞变化，使我们能够同时检测和区分黏附斑和细胞扩展。此外，基于 Al 纳米狭缝的生物传感器芯片还用于评估药物对癌细胞扩展的抑制作用。我们是第一个报告使用双层 Al 纳米狭缝生物传感器检测细胞行为的，这些设备将来可能成为抗转移药物筛选的有力工具。
