Aflatoxin B adsorption/desorption dynamics in the presence of Lactobacillus rhamnosus RC007 in a gastrointestinal tract-simulated model.

AIMS

(i) To determine the aflatoxin B (AFB ) adsorption and desorption dynamics in the presence of Lactobacillus rhamnosus RC007 under simulated transit of AFB at each gastrointestinal tract (GIT-saliva, stomach and intestine) stage consecutively and then, separately, (ii) to study the ability of L. rhamnosus RC007 to biotransform AFB as a strategy that complements the adsorption process.

METHODS AND RESULTS

The AFB adsorption and desorption assay simulating the GIT passage of AFB (93·89 ng g ) in the presence of L. rhamnosus RC007 (10 CFU per ml) was conducted. Moreover, lactic acid production was determined. Results demonstrated that predominant environmental conditions in salivary solution induced a low AFB adsorption, while the transit through the gastric solution and intestinal solution allowed high percentages of adsorption and did not generate significant AFB desorption.

CONCLUSIONS

The AFB adsorption and desorption dynamics in the presence of L. rhamnosus RC007 was favoured by gastric and intestinal environment.

SIGNIFICANCE AND IMPACT OF THE STUDY

The knowledge of the adsorption dynamics of AFB with a micro-organism of interest will allow predicting its behaviour at each stage of the GIT.