Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Multi-Med Plus' Medical Centre in Lodz, Lodz, Poland.
J Cell Mol Med. 2018 Dec;22(12):6386-6390. doi: 10.1111/jcmm.13862. Epub 2018 Sep 6.
The blood platelets are multifunctional blood cells which are involved in the initiation of atheroma, endothelial dysfunction, and modulation of inflammatory and immune responses in the pathophysiology of many diseases. Because of their multifaceted pro-inflammatory activity, platelets may be involved in the pathogenesis of autoimmune thyroid diseases (AITDs), such as Hashimoto's thyroiditis and Graves' disease. The aim of this study was to assess the level of activation and response ability of platelets in AITDs. We used the flow cytometry technique and kinetic measurement of aggregation to analyse platelet function immediately after blood collection and to demonstrate their activation in the circulation of patients with AITDs. We noted reorganization of platelet subpopulations (normal platelets, microparticles and aggregates) in AITDs, dependent on the degree of cell activation. We proved the elevated expression of the active form of integrin receptor GPIIb/IIIa, responsible for platelet aggregation, and in the kinetic test we confirmed the increased aggregation of platelets in different intracellular signal pathways (dependent on ADP, collagen, arachidonic acid). Our study demonstrates the high platelet activation level found in AITDs.
血小板是多功能血细胞，参与动脉粥样硬化的发生、内皮功能障碍以及许多疾病病理生理学中的炎症和免疫反应的调节。由于其多方面的促炎活性，血小板可能参与自身免疫性甲状腺疾病（AITD）的发病机制，如桥本甲状腺炎和格雷夫斯病。本研究旨在评估 AITD 中血小板的激活和反应能力水平。我们使用流式细胞术技术和聚集的动力学测量，在采血后立即分析血小板功能，并在 AITD 患者的循环中证明其激活。我们注意到血小板亚群（正常血小板、微粒体和聚集物）在 AITD 中的重新组织，这取决于细胞激活的程度。我们证明了整合素受体 GPIIb/IIIa 的活性形式的表达升高，该受体负责血小板聚集，并且在动力学测试中我们证实了不同细胞内信号通路（依赖于 ADP、胶原、花生四烯酸）中血小板聚集的增加。我们的研究表明 AITD 中存在高水平的血小板激活。
