Hacettepe University, Faculty of Pharmacy, Department of Pharmacognosy, Sıhhiye, Ankara, 06100, Turkey.
Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry, Sıhhiye, Ankara, 06100, Turkey.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 1;1096:223-227. doi: 10.1016/j.jchromb.2018.08.020. Epub 2018 Aug 21.
Papaver species, well known for their alkaloids, have been used for the treatment of several diseases, such as inflammation, diarrhea, depression, and sleep disorders in certain parts of Anatolia. In this study, four Papaver species (P. lacerum, P. syriacum, P. glaucum and P. rhoeas) were collected from different localities of Turkey. Methanolic extracts were prepared from the aerial parts of the plants. A rapid analytical method was developed for the simultaneously quantitative analysis of two alkaloids, pronuciferine and roemerine, using liquid chromatography tandem mass spectrometry. Multiple reaction monitoring in the positive ionization mode was used for detection. Pronuciferine and roemerine were analyzed on a C column (2.1 × 50 mm, 3 μm) with the mobile phase run in the gradient mode with 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile at a flow rate of 0.3 mL/min. The transitions 312.1→283.1 m/z and 280.0→249.0 m/z were used to monitor pronuciferine and roemerine, respectively. The assay was linear in the concentration range of 0.01 μg/mL to 1 μg/mL (r = 0.996 for roemerine, r = 0.998 for pronuciferine). The validation studies revealed that the method was linear, sensitive, accurate, precise, selective, repeatable, robust, and rugged. Finally, the developed method was applied to quantify pronuciferine and roemerine in the selected species. The amounts of pronuciferine and roemerine were respectively found as 8.5 to 48 μg/g and 4.4 to 43,000 μg/g.
罂粟属植物以其生物碱而闻名，已被用于治疗几种疾病，如炎症、腹泻、抑郁和睡眠障碍，在安纳托利亚的某些地区。在这项研究中，从土耳其的不同地区采集了四种罂粟属植物（P. lacerum、P. syriacum、P. glaucum 和 P. rhoeas）。从植物的地上部分制备了甲醇提取物。建立了一种快速分析方法，用于同时定量分析两种生物碱，即普罗那非林和罗美林，使用液相色谱串联质谱法。在正离子模式下采用多反应监测进行检测。普罗那非林和罗美林在 C 柱（2.1×50mm，3μm）上进行分析，流动相在梯度模式下以水（A）中的 0.1%甲酸和乙腈中的 0.1%甲酸为流动相，流速为 0.3ml/min。分别使用 312.1→283.1 m/z 和 280.0→249.0 m/z 的转换来监测普罗那非林和罗美林。罗美林的测定在 0.01μg/ml 至 1μg/ml 的浓度范围内呈线性（r=0.996，r=0.998）。验证研究表明，该方法具有线性、灵敏、准确、精密、选择性、重复性、稳健性和耐用性。最后，该方法应用于定量分析所选物种中的普罗那非林和罗美林。普罗那非林和罗美林的含量分别为 8.5 至 48μg/g 和 4.4 至 43000μg/g。
