Fabre N, Claparols C, Richelme S, Angelin M L, Fourasté I, Moulis C
Laboratoire Pharmacophores Redox, Phytochimie et Radiobiologie, Université Paul Sabatier, Faculté des Sciences Pharmaceutiques, Toulouse, France.
J Chromatogr A. 2000 Dec 22;904(1):35-46. doi: 10.1016/s0021-9673(00)00919-5.
An ion-pair HPLC-ESI-MS-MS method has been developed for the direct and rapid characterization of isoquinoline alkaloids in a crudely purified extract of the aerial parts of Eschscholtzia californica (Papaveraceae). This plant was chosen because of its increasing use in pharmaceutical industries and because its well known alkaloid composition allows the optimization of the experimental procedure through an on-line analytical sequence. Thus, 14 isoquinoline alkaloids of different types were detected and characterized. The identities of these compounds were confirmed unambigously by their fragmentation and UV spectra obtained by LC-diode-array detection. Various experiments including tandem mass spectrometry and in-orifice collision induced dissociation were performed and prove that MS-MS is a very efficient technique to identify these compounds. An explanation for each isoquinoline alkaloid type MS-MS fragmentation pattern is proposed and indicates similar neutral and/or radical losses. The order of the fragmentation depended on the type of compound but the lost fragments were similar.
已开发出一种离子对高效液相色谱-电喷雾串联质谱法，用于直接快速表征加利福尼亚花菱草（罂粟科）地上部分粗提物中的异喹啉生物碱。选择这种植物是因为其在制药行业中的应用日益广泛，且其众所周知的生物碱组成使得可以通过在线分析序列优化实验程序。因此，检测并表征了14种不同类型的异喹啉生物碱。通过液相色谱-二极管阵列检测获得的碎片和紫外光谱，明确证实了这些化合物的身份。进行了包括串联质谱和孔内碰撞诱导解离在内的各种实验，证明串联质谱是鉴定这些化合物的非常有效的技术。针对每种异喹啉生物碱类型的串联质谱碎片模式提出了解释，表明存在相似的中性和/或自由基损失。碎片的顺序取决于化合物的类型，但丢失的碎片相似。
