Doctorado en Biotecnología, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Edificio 224, Bogotá 110111, Colombia.
Grupo Proteoma UD, Facultad de Ciencias y Educación, Universidad Distrital Francisco José de Caldas, Carrera 4 No. 26B-54, Bogotá 110111, Colombia.
Molecules. 2018 Sep 5;23(9):2262. doi: 10.3390/molecules23092262.
The rice hoja blanca virus (RHBV), transmitted by the planthopper insect , is a disease that attacks rice and generates significant production losses in Colombia. Fedearroz 2000 and Colombia I commercial rice varieties, which have different resistance levels to the disease, were selected in this study. To identify proteins associated to the insect and virus signaling, a comparative proteomics study was performed. By comparing proteomic profiles, between virus-infected and control group plants in two-dimensional electrophoresis, proteins exhibiting significant changes in abundance were found. In another test, peptide dendrimers containing sequences conformationally restricted to α-helix from four of those rice proteins were synthesized. In the experiment, sera from mice inoculated with peptide dendrimers could recognize the corresponding native protein in ELISA assays. Reported comparative proteomic results provide new insights into the molecular mechanisms of plant response to the RHBV and comprehensive tools for the analysis of new crop varieties. Besides, results from conformational peptide dendrimer approach are promising and show that it is feasible to detect proteins as markers, and may have biological applications by decreasing the susceptibility to proteolytic degradation.
水稻黄单胞菌病毒（RHBV）通过叶蝉传播，是一种攻击水稻并在哥伦比亚造成重大产量损失的疾病。本研究选择了 Fedearroz 2000 和 Colombia I 商业水稻品种，它们对该疾病具有不同的抗性水平。为了鉴定与昆虫和病毒信号相关的蛋白质，进行了比较蛋白质组学研究。通过比较感染病毒和对照植物在二维电泳中的蛋白质组图谱，发现了丰度发生显著变化的蛋白质。在另一个测试中，合成了含有来自这四种水稻蛋白的α-螺旋构象限制序列的肽树突状聚合物。在实验中，用肽树突状聚合物接种的小鼠的血清可以在 ELISA 测定中识别相应的天然蛋白。报道的比较蛋白质组学结果为植物对 RHBV 反应的分子机制提供了新的见解，并为分析新的作物品种提供了全面的工具。此外，构象肽树突状聚合物方法的结果很有前景，表明通过降低对蛋白水解降解的敏感性，检测作为标记的蛋白质是可行的，并且可能具有生物学应用。
