Kennedy S, Rice D A, Cush P F
Vet Pathol. 1986 Jul;23(4):454-61. doi: 10.1177/030098588602300416.
Twenty pigs were fed a diet containing 187.5 mg kg-1 of 3-nitro-4-hydroxyphenylarsonic acid (3-nitro). Ten pigs were euthanized at intervals up to 29 days, 3-nitro was withdrawn from the diet of the remaining pigs on day 30, and these animals were subsequently euthanized at intervals up to 49 days after commencement of the experiment. A nervous syndrome characterized by clonic convulsive episodes inducible by exercise, developed at day 11. Paraparesis was apparent at day 22 progressing to paraplegia by day 33 (3 days after cessation of 3-nitro feeding). Histopathologic examination revealed myelin and axonal degeneration in the white matter of the spinal cord coincident with the onset of nervous signs. Marchi-positive degeneration was present in the dorsal funiculus at cervical level at day 22. Lesions intensified with increasing duration of toxicosis and while degenerate fibers were seen in all funiculi, there was preferential involvement of the fasciculi gracilis and cuneatus, the peripheral regions of the ventral and lateral funiculi, and a discrete area of the dorsal region of the lateral funiculus. Peripheral and optic neuropathies were evident from day 32 but were always mild and focal. The experiment establishes 3-nitro as a central-peripheral neurotoxicant of pigs.
给20头猪喂食含有187.5毫克/千克3-硝基-4-羟基苯胂酸（3-硝基）的日粮。在长达29天的时间里，每隔一段时间对10头猪实施安乐死，在第30天从其余猪的日粮中撤去3-硝基，随后在实验开始后长达49天的时间里，每隔一段时间对这些动物实施安乐死。在第11天出现了一种以运动诱发阵挛性惊厥发作为特征的神经综合征。在第22天出现轻瘫，到第33天（停止喂食3-硝基3天后）发展为截瘫。组织病理学检查显示，脊髓白质中的髓鞘和轴突变性与神经症状的出现同时发生。在第22天，颈段背侧索出现马基氏阳性变性。随着中毒时间的延长，病变加剧，虽然在所有索中都可见到变性纤维，但薄束、楔束、腹侧索和外侧索的周边区域以及外侧索背侧区域的一个离散区域优先受累。从第32天开始出现周围神经和视神经病变，但始终较轻且为局灶性。该实验确定3-硝基是猪的一种中枢-外周神经毒物。
