Department of Chemistry, University of AJK, Muzaffarabad 13100, Pakistan.
Department of Botany, University of AJK, Muzaffarabad 13100, Pakistan.
J Nanosci Nanotechnol. 2018 Dec 1;18(12):8386-8391. doi: 10.1166/jnn.2018.16401.
Green syntheses of nanoparticles using plant materials are of tremendous scope. Here we report advantageous green synthesis for silver nanoparticles (AgNPs) using aqueous-root extract of Jurinea dolomiaea and AgNO3. Color change of solution and UV-Vis absorption at 444 nm indicated the formation of AgNPs. XRD confirmed their face centered cubic structure (fcc) with average particle size of 24.58 nm. SEM analysis showed their spherical, cubic and triangular structures. FT-IR indicated the presence of functional groups of reducing and stabilizing phytochemicals. Methanol-root extract of J. dolomiaea revealed high flavonoid (445 mg RE/g) and phenolic contents (92 mg GAE/g). Methanol-extract showed high antioxidant potency (IC50 = 0.494 μg/mL), rationally due to its high phenolic and flavonoid contents. These AgNPs showed the highest and equal antimicrobial activities against Escherichia coli and Pseudomonas aeruginosa (Inhibition zone 11.0 mm) whereas, methanol-roots extract showed equal and intermediate activities (Inhibition zone 8.0 mm) against both pathogens but aqueous extract showed poor activities (Inhibition zone 6.0 mm) against these both pathogens. AgNPs are playing a major role in the field of nanotechnology and nanomedicine due to their antimicrobial and drug delivery efficacy as well as reasonable tolerance in human biology.
利用植物材料进行纳米粒子的绿色合成具有巨大的潜力。在这里，我们报告了一种使用 Jurinea dolomiaea 的水生根提取物和 AgNO3 进行银纳米粒子（AgNPs）的有利绿色合成的方法。溶液颜色的变化和在 444nm 处的紫外-可见吸收表明 AgNPs 的形成。XRD 证实了它们具有面心立方结构（fcc），平均粒径为 24.58nm。SEM 分析表明它们具有球形、立方和三角形结构。FT-IR 表明存在具有还原和稳定植物化学物质功能的官能团。J. dolomiaea 的甲醇根提取物显示出高含量的类黄酮（445mgRE/g）和酚类（92mgGAE/g）。甲醇提取物表现出高抗氧化活性（IC50=0.494μg/mL），这是由于其高酚类和类黄酮含量。这些 AgNPs 对大肠杆菌和铜绿假单胞菌表现出最高和相等的抗菌活性（抑菌圈 11.0mm），而甲醇根提取物对两种病原体均表现出相等和中等的活性（抑菌圈 8.0mm），但水提取物对这两种病原体的活性较差（抑菌圈 6.0mm）。AgNPs 由于其在人类生物学中的抗菌和药物输送功效以及合理的耐受性，在纳米技术和纳米医学领域发挥着重要作用。
