Department of Chemistry, University of AJK, Muzaffarabad 13100, Pakistan.
Department of Biology, Lahore University of Management Science, Lahore 54792, Pakistan.
J Nanosci Nanotechnol. 2018 Dec 1;18(12):8392-8398. doi: 10.1166/jnn.2018.16414.
Nanotechnology has become an irreplaceable need and green synthesis of nanoparticles offers several advantages over physical and chemical methods. Medicinal plants are the main reservoirs of drugs and drug candidates. We report the biogenic synthesis of silver nanoparticles (AgNPs) using aqueous root extract of Saussurea lappa. Verification and characterization of these nanoparticles were done by UV-visible spectroscopy, XRD-analysis and Scanning Electron Microscopy and FT-IR. Extract-loaded-AgNPs showed the highest inhibition zone against Escherichia coli (11.0 mm) and intermediate against Pseudomonas aeruginosa (9.0 mm). The methanolic root extract of S. lappa alone, also moderately inhibited Pseudomonas aeruginosa (9.0 mm) and showed lower activity (6.0 mm) against Escherichia coli. Its aqueous roots extract inhibited (6.0 mm) the growth of tested organisms. Methanolic extract showed antioxidant potency (IC50 = 0.814 μg/mL). Experiments revealed the presence of phenols and flavonoids in the roots of Saussurea lappa. These findings provide promising interest to exploit Saussurea lappa for the biogenic synthesis of AgNPs and their biological applications.
纳米技术已经成为一种不可或缺的需求，而纳米粒子的绿色合成相对于物理和化学方法具有多项优势。药用植物是药物和候选药物的主要来源。我们报告了使用西藏雪莲的水根提取物生物合成银纳米粒子（AgNPs）。通过紫外可见光谱、XRD 分析、扫描电子显微镜和傅里叶变换红外光谱对这些纳米粒子进行了验证和表征。负载提取物的 AgNPs 对大肠杆菌的抑制圈最高（11.0 毫米），对铜绿假单胞菌的抑制作用中等（9.0 毫米）。西藏雪莲的甲醇根提取物本身也适度抑制铜绿假单胞菌（9.0 毫米），对大肠杆菌的活性较低（6.0 毫米）。其水根提取物抑制了（6.0 毫米）测试生物的生长。甲醇提取物表现出抗氧化能力（IC50 = 0.814 μg/mL）。实验表明，西藏雪莲的根中存在酚类和类黄酮。这些发现为利用西藏雪莲进行 AgNPs 的生物合成及其生物应用提供了有前途的研究兴趣。
