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1
Molecular size of N-acetylglucosaminylphosphotransferase and alpha-N-acetylglucosaminyl phosphodiesterase as determined in situ in Golgi membranes by radiation inactivation.通过辐射失活原位测定高尔基体膜中N-乙酰葡糖胺磷酸转移酶和α-N-乙酰葡糖胺磷酸二酯酶的分子大小。
Biochem J. 1986 May 1;235(3):883-6. doi: 10.1042/bj2350883.
2
Subfractionation of rat liver Golgi apparatus: separation of enzyme activities involved in the biosynthesis of the phosphomannosyl recognition marker in lysosomal enzymes.大鼠肝脏高尔基体的亚分级分离：溶酶体酶中磷酸甘露糖识别标记生物合成相关酶活性的分离
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3938-42. doi: 10.1073/pnas.80.13.3938.
3
Synthesis of phosphorylated recognition marker in lysosomal enzymes is located in the cis part of Golgi apparatus.溶酶体酶中磷酸化识别标记的合成位于高尔基体的顺面部分。
J Biol Chem. 1982 May 25;257(10):5323-5.
4
Fibroblasts from patients with I-cell disease and pseudo-Hurler polydystrophy are deficient in uridine 5'-diphosphate-N-acetylglucosamine: glycoprotein N-acetylglucosaminylphosphotransferase activity.患有I型细胞病和假胡尔勒氏多营养不良症患者的成纤维细胞缺乏尿苷5'-二磷酸-N-乙酰葡糖胺：糖蛋白N-乙酰葡糖胺磷酸转移酶活性。
J Clin Invest. 1981 May;67(5):1574-9. doi: 10.1172/jci110189.
5
Radiometric assays of N-acetylglucosaminylphosphotransferase and alpha-N-acetylglucosaminyl phosphodiesterase with substrates labeled in the glucosamine moiety.用葡糖胺部分标记的底物对N-乙酰葡糖胺磷酸转移酶和α-N-乙酰葡糖胺磷酸二酯酶进行放射性测定。
Anal Biochem. 1984 Nov 1;142(2):297-304. doi: 10.1016/0003-2697(84)90468-8.
6
Steps in the phosphorylation of the high mannose oligosaccharides of lysosomal enzymes.溶酶体酶高甘露糖寡糖磷酸化的步骤。
Ciba Found Symp. 1982(92):138-56. doi: 10.1002/9780470720745.ch8.
7
Characterization of the mutant N-acetylglucosaminylphosphotransferase in I-cell disease and pseudo-Hurler polydystrophy: complementation analysis and kinetic studies.I型细胞病和假性胡尔勒多营养不良中突变型N-乙酰葡糖胺磷酸转移酶的特征：互补分析和动力学研究。
Enzyme. 1986;35(2):106-16. doi: 10.1159/000469330.
8
Lysosomal enzyme targeting. N-Acetylglucosaminylphosphotransferase selectively phosphorylates native lysosomal enzymes.溶酶体酶靶向作用。N-乙酰葡糖胺磷酸转移酶选择性地使天然溶酶体酶磷酸化。
J Biol Chem. 1981 Dec 10;256(23):11977-80.
9
Identification of a rat liver alpha-N-acetylglucosaminyl phosphodiesterase capable of removing "blocking" alpha-N-acetylglucosamine residues from phosphorylated high mannose oligosaccharides of lysosomal enzymes.一种能够从溶酶体酶的磷酸化高甘露糖寡糖中去除“封闭性”α-N-乙酰葡糖胺残基的大鼠肝脏α-N-乙酰葡糖胺磷酸二酯酶的鉴定。
J Biol Chem. 1980 Sep 25;255(18):8398-401.
10
Human beta-glucuronidase pinocytosis and binding to the immobilized phosphomannosyl receptor. Effects of treatment of the enzyme with alpha-N-acetylglucosaminyl phosphodiesterase.人β-葡萄糖醛酸酶的胞饮作用及其与固定化磷酸甘露糖受体的结合。用α-N-乙酰葡糖胺基磷酸二酯酶处理该酶的效果。
J Biol Chem. 1983 Jun 25;258(12):7345-51.

引用本文的文献

1
Altered molecular size of N-acetylglucosamine 1-phosphotransferase in I-cell disease and pseudo-Hurler polydystrophy.I型细胞病和假胡尔勒氏多营养不良中N-乙酰葡糖胺1-磷酸转移酶分子大小的改变。
Biochem J. 1987 Dec 15;248(3):697-701. doi: 10.1042/bj2480697.
2
Mucolipidoses II and III variants with normal N-acetylglucosamine 1-phosphotransferase activity toward alpha-methylmannoside are due to nonallelic mutations.对α-甲基甘露糖苷具有正常N-乙酰葡糖胺1-磷酸转移酶活性的II型和III型黏脂贮积症变体是由非等位基因突变引起的。
Am J Hum Genet. 1992 Jan;50(1):137-44.

本文引用的文献

1
A method for rapid isolation of rough and smooth microsomes and Golgi apparatus from rat liver in the same sucrose gradient.一种在相同蔗糖梯度中从大鼠肝脏快速分离粗糙型和光滑型微粒体及高尔基体的方法。
Exp Cell Res. 1980 Dec;130(2):393-400. doi: 10.1016/0014-4827(80)90017-8.
2
Radiation inactivation of enzymes at low dose rates: identical molecular weights of rat liver cytosolic and lysosomal neuraminidases.低剂量率下酶的辐射失活：大鼠肝脏胞质和溶酶体神经氨酸酶的分子量相同
Anal Biochem. 1982 May 15;122(2):379-84. doi: 10.1016/0003-2697(82)90299-8.
3
Mucolipidosis III is genetically heterogeneous.黏脂贮积症III型在遗传上具有异质性。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7420-4. doi: 10.1073/pnas.79.23.7420.
4
Target size analysis by radiation inactivation: a large capacity tube rack for irradiation in a Gammacell 220.通过辐射失活进行靶标大小分析：用于在伽马细胞220中进行辐照的大容量试管架。
Anal Biochem. 1983 Jul 15;132(2):362-4. doi: 10.1016/0003-2697(83)90021-0.
5
Identification of a variant of mucolipidosis III (pseudo-Hurler polydystrophy): a catalytically active N-acetylglucosaminylphosphotransferase that fails to phosphorylate lysosomal enzymes.黏脂贮积症III型（假胡尔勒多营养不良症）一种变异型的鉴定：一种具有催化活性但无法磷酸化溶酶体酶的N-乙酰葡糖胺磷酸转移酶。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7773-7. doi: 10.1073/pnas.78.12.7773.
6
Lysosomal enzyme targeting. N-Acetylglucosaminylphosphotransferase selectively phosphorylates native lysosomal enzymes.溶酶体酶靶向作用。N-乙酰葡糖胺磷酸转移酶选择性地使天然溶酶体酶磷酸化。
J Biol Chem. 1981 Dec 10;256(23):11977-80.
7
Enzymatic phosphorylation of lysosomal enzymes in the presence of UDP-N-acetylglucosamine. Absence of the activity in I-cell fibroblasts.在UDP-N-乙酰葡糖胺存在的情况下溶酶体酶的酶促磷酸化。I型细胞成纤维细胞中缺乏该活性。
Biochem Biophys Res Commun. 1981 Feb 12;98(3):761-7. doi: 10.1016/0006-291x(81)91177-3.
8
Mucolipidosis II and III. The genetic relationships between two disorders of lysosomal enzyme biosynthesis.黏脂贮积症II型和III型。溶酶体酶生物合成的两种疾病之间的遗传关系。
J Clin Invest. 1983 Sep;72(3):1016-23. doi: 10.1172/JCI111025.
9
UDP-N-acetylglucosamine:lysosomal enzyme precursor N-acetylglucosamine-1-phosphotransferase. Partial purification and characterization of the rat liver Golgi enzyme.UDP-N-乙酰葡糖胺：溶酶体酶前体N-乙酰葡糖胺-1-磷酸转移酶。大鼠肝脏高尔基体酶的部分纯化及特性鉴定
J Biol Chem. 1982 Oct 25;257(20):12322-31.
10
Genetic heterogeneity of I-cell disease is demonstrated by complementation of lysosomal enzyme processing mutants.溶酶体酶加工突变体的互补作用证明了I-细胞病的遗传异质性。
Am J Med Genet. 1982 Jul;12(3):343-53. doi: 10.1002/ajmg.1320120312.

通过辐射失活原位测定高尔基体膜中N-乙酰葡糖胺磷酸转移酶和α-N-乙酰葡糖胺磷酸二酯酶的分子大小。

Molecular size of N-acetylglucosaminylphosphotransferase and alpha-N-acetylglucosaminyl phosphodiesterase as determined in situ in Golgi membranes by radiation inactivation.

作者信息

Ben-Yoseph Y, Potier M, Pack B A, Mitchell D A, Melançon S B, Nadler H L

出版信息

Biochem J. 1986 May 1;235(3):883-6. doi: 10.1042/bj2350883.

DOI:10.1042/bj2350883
PMID:3019310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146769/
Abstract

The radiation inactivation method was used to determine the molecular size of the two enzymes that participate in the synthesis of the phosphomannosyl recognition marker of lysosomal proteins. The determinations were carried out in situ, in Golgi membranes isolated from normal human placenta and cultured skin fibroblasts. A molecular size of 228 +/- 29 kDa was found for placental N-acetylglucosaminyl-phosphotransferase, and 129 +/- 11 kDa for placental alpha-N-acetylglucosaminyl phosphodiesterase. The values for the fibroblast enzymes were about 20% higher, 283 +/- 27 kDa and 156 +/- 14 kDa for the transferase and phosphodiesterase respectively. Triton X-100 had no effect on the molecular size of these enzymes.

摘要

采用辐射失活法测定参与溶酶体蛋白磷酸甘露糖识别标记合成的两种酶的分子大小。测定是在原位进行的，所用的高尔基体膜取自正常人胎盘和培养的皮肤成纤维细胞。胎盘N-乙酰葡糖胺磷酸转移酶的分子大小为228±29 kDa，胎盘α-N-乙酰葡糖胺磷酸二酯酶的分子大小为129±11 kDa。成纤维细胞中这两种酶的值分别约高20%，转移酶为283±27 kDa，磷酸二酯酶为156±14 kDa。Triton X-100对这些酶的分子大小没有影响。