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A method for rapid isolation of rough and smooth microsomes and Golgi apparatus from rat liver in the same sucrose gradient.

作者信息

Sandberg P O, Marzella L, Glaumann H

出版信息

Exp Cell Res. 1980 Dec;130(2):393-400. doi: 10.1016/0014-4827(80)90017-8.

DOI:10.1016/0014-4827(80)90017-8
PMID:7449858
Abstract
摘要

相似文献

1
A method for rapid isolation of rough and smooth microsomes and Golgi apparatus from rat liver in the same sucrose gradient.一种在相同蔗糖梯度中从大鼠肝脏快速分离粗糙型和光滑型微粒体及高尔基体的方法。
Exp Cell Res. 1980 Dec;130(2):393-400. doi: 10.1016/0014-4827(80)90017-8.
2
Isolation and characterization of Golgi apparatus and membranes from rat liver.大鼠肝脏高尔基体及膜的分离与鉴定
Methods Enzymol. 1974;31:180-91. doi: 10.1016/0076-6879(74)31020-8.
3
Isolation of Golgi apparatus from rat liver using D2O-sucrose gradients.使用重水 - 蔗糖梯度从大鼠肝脏中分离高尔基体。
Methods Enzymol. 1983;98:60-7. doi: 10.1016/0076-6879(83)98139-9.
4
Separation of the intracellular secretory compartment of rat liver and isolated rat hepatocytes in a single step using self-generating gradients of iodixanol.利用碘克沙醇的自生成梯度一步分离大鼠肝脏和分离的大鼠肝细胞的细胞内分泌区室。
Anal Biochem. 1999 Dec 1;276(1):88-96. doi: 10.1006/abio.1999.4311.
5
Subcellular fractionation of rat liver.大鼠肝脏的亚细胞分级分离
Methods Enzymol. 1974;31:6-41. doi: 10.1016/0076-6879(74)31005-1.
6
Preparation of membrane fractions.膜组分的制备。
Methods Enzymol. 1990;182:225-35. doi: 10.1016/0076-6879(90)82019-x.
7
Sequential preparation of rat liver microsomal and Golgi membranes.大鼠肝微粒体和高尔基体膜的顺序制备
Biochim Biophys Acta. 1978 Oct 4;512(3):539-49. doi: 10.1016/0005-2736(78)90163-3.
8
The synthesis of rat liver lysosomes. I. Comparison of the microsomal, golgi, and lysosomal beta-glucuronidases.大鼠肝脏溶酶体的合成。I.微粒体、高尔基体和溶酶体β-葡萄糖醛酸酶的比较。
J Biochem. 1977 Sep;82(3):619-36. doi: 10.1093/oxfordjournals.jbchem.a131737.
9
Recovery of galactosyltransferase activity from sucrose gradients during isolation of Golgi membranes.在高尔基体膜分离过程中从蔗糖梯度中回收半乳糖基转移酶活性。
J Membr Biol. 1974;19(4):397-408. doi: 10.1007/BF01869988.
10
Endogenous localization of UDP-galactose:asialomucin galactosyltransferase activity in rat liver endoplasmic reticulum and Golgi apparatus.大鼠肝脏内质网和高尔基体中UDP-半乳糖:去唾液酸糖蛋白半乳糖基转移酶活性的内源性定位
J Biol Chem. 1981 Sep 25;256(18):9633-9.

引用本文的文献

1
Importance of glycolipid synthesis for butyric acid-induced sensitization to shiga toxin and intracellular sorting of toxin in A431 cells.糖脂合成对丁酸诱导的A431细胞对志贺毒素致敏作用及毒素细胞内分选的重要性。
Mol Biol Cell. 1996 Sep;7(9):1391-404. doi: 10.1091/mbc.7.9.1391.
2
Isolation of three novel cholinergic neuron-specific gangliosides from bovine brain and their in vitro syntheses.从牛脑中分离出三种新型胆碱能神经元特异性神经节苷脂及其体外合成。
Glycoconj J. 1996 Apr;13(2):177-86. doi: 10.1007/BF00731492.
3
Cholesterol-containing lactose derived neoglycolipids serve as acceptors for sialyltransferases from rat liver Golgi vesicles.
Glycoconj J. 1996 Apr;13(2):147-52. doi: 10.1007/BF00731488.
4
Retrograde transport from the Golgi complex to the ER of both Shiga toxin and the nontoxic Shiga B-fragment is regulated by butyric acid and cAMP.从高尔基体复合体到内质网的志贺毒素和无毒志贺B片段的逆行转运受丁酸和环磷酸腺苷调节。
J Cell Biol. 1994 Jul;126(1):53-64. doi: 10.1083/jcb.126.1.53.
5
Sheep pancreatic microsomes as an alternative to the dog source for studying protein translocation.绵羊胰腺微粒体作为研究蛋白质转运的犬源替代物。
Biochem J. 1995 Feb 15;306 ( Pt 1)(Pt 1):57-61. doi: 10.1042/bj3060057.
6
Cleavage of honeybee prepromelittin by an endoprotease from rat liver microsomes: identification of intact signal peptide.大鼠肝微粒体中的一种内切蛋白酶对蜜蜂前原蜂毒肽的切割:完整信号肽的鉴定。
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2260-3. doi: 10.1073/pnas.79.7.2260.
7
Tunicamycin inhibits ganglioside biosynthesis in rat liver Golgi apparatus by blocking sugar nucleotide transport across the membrane vesicles.衣霉素通过阻断糖核苷酸跨膜囊泡转运来抑制大鼠肝脏高尔基体中的神经节苷脂生物合成。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7075-9. doi: 10.1073/pnas.80.23.7075.
8
Incorporation and metabolism of exogenous GM1 ganglioside in rat liver.外源性GM1神经节苷脂在大鼠肝脏中的掺入与代谢
Biochem J. 1986 Jul 1;237(1):147-55. doi: 10.1042/bj2370147.
9
Different oligosaccharide processing of the membrane-integrated and the secretory form of gp 80 in rat liver.
EMBO J. 1986 Sep;5(9):2109-14. doi: 10.1002/j.1460-2075.1986.tb04473.x.
10
Both GA2, GM2, and GD2 synthases and GM1b, GD1a, and GT1b synthases are single enzymes in Golgi vesicles from rat liver.GA2、GM2和GD2合酶以及GM1b、GD1a和GT1b合酶在大鼠肝脏的高尔基体囊泡中均为单一酶。
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7044-8. doi: 10.1073/pnas.85.19.7044.