Tian Q, Feng Y, Huang H, Zhang J, Yu Y, Guan Z, Cai Y, Liao X
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
Lett Appl Microbiol. 2018 Dec;67(6):589-597. doi: 10.1111/lam.13070. Epub 2018 Nov 12.
The aim of this study was to produce lactobionic acid from lactose by a new Pycnoporus sp. SYBC-L10 strain. Recently, studies on enzymatic production of lactobionic acid mostly focus on cellobiose dehydrogenase from Sclerotium rolfsii CBS 191·62 and laccase from Trametes pubescens MB 89 oxidize lactose to lactobionic acid with redox mediators. In this study, we converted lactose to lactobionic acid by shaking flask fermentation without exogenous mediator in the reaction mixture. In this bioconversion process, lactose is efficiently converted into lactobionic acid with a specific productivity of up to 3·1 g l h and 96% yield. 3-Hydroxyanthranilic acid added externally to the reaction mixture can obviously accelerate the conversion of lactose to lactobionic acid. The results showed that 3-hydroxyanthranilic acid produced by the fungus itself is an important influencing factor in this bioconversion process. This study presents the first attempt to efficiently produce lactobionic acid by white-rot fungi, suggesting definite potential for Pycnoporus to produce lactobionic acid. SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobionic acid has been applied to a wide range of applications in pharmaceutical, food, nanotechnology and chemical industries. Here, an attempt was done to produce lactobionic acid from lactose using the cellobiose dehydrogenase-3-HAA-laccase system in a fermentation system. After a survey of other methods to produce lactobionic acid by cellobiose dehydrogenase, this study explores a new and significant perspective for the production of lactobionic acid.
本研究的目的是利用一种新的多孔菌属SYBC-L10菌株从乳糖中生产乳糖酸。最近，关于乳糖酸酶促生产的研究大多集中在罗氏白僵菌CBS 191·62的纤维二糖脱氢酶和柔毛栓菌MB 89的漆酶通过氧化还原介质将乳糖氧化为乳糖酸。在本研究中，我们通过摇瓶发酵在反应混合物中不添加外源介质的情况下将乳糖转化为乳糖酸。在这个生物转化过程中，乳糖能高效地转化为乳糖酸，比生产率高达3.1 g/(L·h)，产率为96%。向反应混合物中外部添加3-羟基邻氨基苯甲酸可以明显加速乳糖向乳糖酸的转化。结果表明，真菌自身产生的3-羟基邻氨基苯甲酸是该生物转化过程中的一个重要影响因素。本研究首次尝试利用白腐真菌高效生产乳糖酸，表明多孔菌属在生产乳糖酸方面具有一定潜力。研究的意义和影响：乳糖酸已在制药、食品、纳米技术和化学工业等广泛领域得到应用。在此，尝试在发酵系统中利用纤维二糖脱氢酶-3-羟基邻氨基苯甲酸-漆酶系统从乳糖中生产乳糖酸。在调研了其他利用纤维二糖脱氢酶生产乳糖酸的方法后，本研究为乳糖酸的生产探索了一个新的重要视角。
