Madelaire Carla Bonetti, Cassettari Bruna de Oliveira, Gomes Fernando Ribeiro
USP - Universidade de São Paulo, Instituto de Biociências, Departamento de Fisiologia, Rua do Matão, trav 14 n 321, Cidade Universitária, São Paulo, SP 05508-090, Brazil.
USP - Universidade de São Paulo, Instituto de Biociências, Departamento de Fisiologia, Rua do Matão, trav 14 n 321, Cidade Universitária, São Paulo, SP 05508-090, Brazil.
Gen Comp Endocrinol. 2019 Mar 1;273:227-235. doi: 10.1016/j.ygcen.2018.09.005. Epub 2018 Sep 5.
Androgens and glucocorticoids play important roles in vertebrate's reproduction and display complex immunomodulatory functions that may affect survival. In anurans, testosterone and corticosterone are correlated to sexual behavior, reproduction, and immune function. Male toads (Rhinella jimi) were treated with acute doses of testosterone (T) and corticosterone (CORT) and immune variables (plasma bacterial killing ability [BKA], swelling after phytohemagglutinin [PHA] injection and the time point of maximum PHA swelling response) were measured. Transdermal T and CORT application increased androgen (T-DHT) and CORT plasma levels after 1 h of treatment, respectively, without a dose-specific effect. Transdermal T treatment did not affect BKA or PHA swelling response. Individuals treated with transdermal CORT showed an earlier maximum PHA swelling response and a tendency of lower BKA 15 h after treatment. Our results indicated that an acute experimental increase of CORT plasma levels diminished time for inflammatory resolution and suppressed non-cellular innate response.
雄激素和糖皮质激素在脊椎动物的繁殖中发挥重要作用，并表现出可能影响生存的复杂免疫调节功能。在无尾两栖类动物中，睾酮和皮质酮与性行为、繁殖及免疫功能相关。对雄性蟾蜍（奇异多指节蟾）给予急性剂量的睾酮（T）和皮质酮（CORT）处理，并测量免疫变量（血浆细菌杀伤能力[BKA]、注射植物血凝素[PHA]后的肿胀情况以及PHA肿胀反应的最大时间点）。经皮应用T和CORT分别在处理1小时后提高了雄激素（T - DHT）和CORT的血浆水平，且无剂量特异性效应。经皮T处理不影响BKA或PHA肿胀反应。经皮CORT处理的个体在处理后15小时显示出PHA肿胀反应的最大时间点提前，且BKA有降低的趋势。我们的结果表明，CORT血浆水平的急性实验性升高缩短了炎症消退时间并抑制了非细胞固有反应。
