Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2018 Dec;28:122-133. doi: 10.1016/j.cbd.2018.08.001. Epub 2018 Sep 1.
Olfaction in antennae is essential for regulating insect behaviors such as host preference and oviposition site selection. To better understand the olfactory mechanisms in the cicada Subpsaltria yangi that has a very narrow host range far fewer than diets of most other cicadas, an antennal transcriptome was constructed in this study. We identified 10 unigenes encoding putative odorant-binding proteins (OBPs), 10 chemosensory proteins (CSPs), 8 sensory neuron membrane proteins (SNMPs), 7 gustatory receptors (GRs), 29 odorant receptors (ORs) and 13 ionotropic receptors (IRs). We found that the OBPs were separated into two groups, Classic OBPs and Plus-C OBPs, according to their motifs. Based on sequence alignment and phylogenetic analysis, we found five Orcos in OR family. However, no pheromone receptor was identified, which may be related to that cicadas are sound-producing insects and acoustic signals other than pheromones play most important role in sexual communication. We used qRT-PCR to examine the expression profile of ten OBPs genes in various organs, and found that they were mainly expressed in olfactory organs such as antennae and mouthparts. Our results make it possible for future research of the olfactory system of S. yangi at the molecular level, and provide important bases for elucidation of the molecular mechanisms and evolution of chemosensation in sap-sucking insects.
触角中的嗅觉对于调节昆虫行为（如宿主偏好和产卵场所选择）至关重要。为了更好地理解蝉科昆虫 Subpsaltria yangi 的嗅觉机制，该昆虫的宿主范围非常狭窄，远少于大多数其他蝉的饮食范围，本研究构建了一个触角转录组。我们鉴定了 10 个编码假定气味结合蛋白 (OBP)、10 个化学感觉蛋白 (CSP)、8 个感觉神经元膜蛋白 (SNMP)、7 个味觉受体 (GR)、29 个气味受体 (OR) 和 13 个离子型受体 (IR)的基因。我们发现，根据其基序，OBPs 可分为经典 OBP 和 Plus-C OBP 两组。基于序列比对和系统发育分析，我们在 OR 家族中发现了五个 Orcos。然而，没有鉴定出信息素受体，这可能与蝉是发声昆虫，声信号而非信息素在性通讯中起着最重要的作用有关。我们使用 qRT-PCR 检测了十种 OBP 基因在各种器官中的表达谱，发现它们主要在嗅觉器官（如触角和口器）中表达。我们的研究结果为 S. yangi 嗅觉系统的未来分子研究奠定了基础，并为阐明吸食汁液昆虫的化学感觉分子机制和进化提供了重要依据。
