Lomonosov Moscow State University, Institute of Regenerative Medicine, Moscow, 119192, Russia.
Lomonosov Moscow State University, Faculty of Fundamental Medicine, Moscow, 119192, Russia.
Biochemistry (Mosc). 2018 Jun;83(6):629-642. doi: 10.1134/S0006297918060020.
CRISPR/Cas9 genome-editing system is a powerful, fairly accurate, and efficient tool for modifying genomic DNA. Despite obvious advantages, it is not devoid of certain drawbacks, such as propensity for introduction of additional nonspecific DNA breaks, insufficient activity against aneuploid genomes, and relative difficulty in delivering its components to cells. In this review, we focus on the difficulties that can limit the use of CRISPR/Cas9 and suggest a number of practical recommendations and information sources that will make it easier for the beginners to work with this outstanding technological achievement of the XXI century.
CRISPR/Cas9 基因组编辑系统是一种强大、相当准确和高效的工具，可用于修饰基因组 DNA。尽管有明显的优势，但它并非没有某些缺点，例如容易引入额外的非特异性 DNA 断裂、对非整倍体基因组的活性不足，以及将其成分递送到细胞的相对困难。在这篇综述中，我们重点介绍了可能限制 CRISPR/Cas9 使用的困难，并提出了一些实用建议和信息来源，这将使初学者更容易使用这一 21 世纪杰出的技术成就。
