来自锥虫线粒体的移码coxII基因的主要转录本包含四个未在DNA中编码的核苷酸。

Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA.

作者信息

Benne R, Van den Burg J, Brakenhoff J P, Sloof P, Van Boom J H, Tromp M C

出版信息

Cell. 1986 Sep 12;46(6):819-26. doi: 10.1016/0092-8674(86)90063-2.

PMID:3019552

Abstract

The mitochondrial cytochrome oxidase (cox) subunit II gene from trypanosomes contains a frameshift at amino acid 170. This gene is highly conserved in different trypanosome species, suggesting that it is functional. Sequence determination of coxII transcripts of T. brucei and C. fasciculata reveals four extra, reading frame-restoring nucleotides at the frameshift position that are not encoded in the DNA. Southern blot analysis of DNA of both trypanosome species failed to show the existence of a second version of the coxII gene. We conclude, therefore, that the extra nucleotides are added during or after transcription of the frameshift gene by an RNA-editing process.

摘要

锥虫的线粒体细胞色素氧化酶（cox）亚基II基因在氨基酸170处存在移码。该基因在不同锥虫物种中高度保守，表明它具有功能。布氏锥虫和 fasciculata锥虫coxII转录本的序列测定显示，在移码位置有四个额外的、恢复读框的核苷酸，这些核苷酸在DNA中没有编码。对这两种锥虫物种的DNA进行Southern印迹分析，未能显示coxII基因的第二个版本的存在。因此，我们得出结论，这些额外的核苷酸是在移码基因转录期间或转录后通过RNA编辑过程添加的。