Conaty Peter, Sherman Lauren S, Naaldijk Yahaira, Ulrich Henning, Stolzing Alexandra, Rameshwar Pranela
Division of Hematology/Oncology, Department of Medicine, New Jersey Medical School, Rutgers School of Biomedical Health Science, Newark, NJ, USA.
Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.
Methods Mol Biol. 2018;1842:81-91. doi: 10.1007/978-1-4939-8697-2_6.
Mesenchymal stem/stromal cells (MSC) are multipotent cells that can be isolated from adult and fetal tissues. In vitro, MSCs show functional plasticity by differentiating into specialized cells of all germ layers. MSCs are of relevant to medicine and have been proposed for several disorders. MSCs can be transplanted across allogeneic barriers as "off the shelf" cells. This chapter focuses on methods to deliver MSCs to the brain because neurological pathology such as damage due to stroke can lead to debilitating mental and physical problems. In general, neurological diseases are difficult to treat, partly due to the challenge in getting drugs across the blood-brain barrier (BBB). MSCs as well as other stem cells can cross the BBB. The described method begins to develop procedures, leading to efficient delivery of drugs to the brain. Here describe how MSCs can be propagated from bone marrow aspirates and their utility in delivering small RNA to the brain. The chapter discusses the issue to enhance efficient delivery of MSCs to the brain.
间充质干/基质细胞（MSC）是一类多能细胞，可从成人和胎儿组织中分离得到。在体外，MSC通过分化为所有胚层的特化细胞表现出功能可塑性。MSC与医学相关，已被应用于多种疾病的治疗。MSC可作为“现成可用”的细胞跨越同种异体屏障进行移植。本章重点介绍将MSC递送至大脑的方法，因为诸如中风导致的损伤等神经病理学情况可引发使人衰弱的精神和身体问题。一般来说，神经疾病难以治疗，部分原因在于药物穿越血脑屏障（BBB）存在挑战。MSC以及其他干细胞能够穿越BBB。所描述的方法开始开发相关程序，以实现向大脑高效递送药物。在此描述如何从骨髓抽吸物中扩增MSC及其在向大脑递送小RNA方面的效用。本章讨论了提高MSC向大脑高效递送的相关问题。
