Georg August University of Goettingen, Institute of Physical Chemistry, Tammannstr. 6, 37077, Goettingen, Germany.
Georg August University of Goettingen, Institute of Physical Chemistry, Tammannstr. 6, 37077, Goettingen, Germany.
Prog Biophys Mol Biol. 2019 Jul;144:77-90. doi: 10.1016/j.pbiomolbio.2018.08.010. Epub 2018 Sep 7.
The F-actin cytoskeleton and its connection to the plasma membrane provide structure and shape of epithelial cells. In this study we focus on the impact of the F-actin cytoskeleton on the morphology and mechanical behaviour of confluent epithelial cells. F-actin depolymerisation was fostered by Latrunculin A, while depolymerisation was allayed by Jasplakinolide. The impact of drug treatment on cellular mechanics was measured using atomic force microscopy based active microrheology and force-indentation curves, while morphology was monitored by AFM imaging, electric cell-substrate impedance sensing (ECIS) experiments and fluorescence microscopy. A softening and fluidisation of the cells upon dissolution of F-actin was observed, accompanied by reduction of cell-substrate and cell-cell contacts and an altered topography. The strengthening of actin filaments upon Jasplakinolide treatment was mirrored in several mechanical properties. The largest impact was on the cellular viscosity. The cells were, however, capable of restoring their initial phenotypes, e.g., amount of actin, intercellular and cell-substrate interactions.
细胞骨架 F-肌动蛋白及其与质膜的连接为上皮细胞提供了结构和形状。在这项研究中，我们专注于 F-肌动蛋白细胞骨架对细胞形态和机械行为的影响。Latrunculin A 促进 F-肌动蛋白解聚，而 Jasplakinolide 则缓解 F-肌动蛋白解聚。使用基于原子力显微镜的主动微流变学和力-压痕曲线测量药物处理对细胞力学的影响，同时通过原子力显微镜成像、细胞-基底阻抗传感 (ECIS) 实验和荧光显微镜监测形态。观察到 F-肌动蛋白溶解后细胞变软和流态化，伴随着细胞-基底和细胞-细胞接触减少以及形貌改变。 Jasplakinolide 处理后肌动蛋白丝的增强反映在几个机械特性上。最大的影响是细胞粘度。然而，细胞能够恢复其初始表型，例如肌动蛋白、细胞间和细胞-基底相互作用的数量。
