Autoradiographic localization of alpha-adrenoceptors, muscarinic acetylcholine receptors and opiate receptors in the heart.

Using in vitro autoradiography the distribution of [3H]rauwolscine, [3H]prazosin and [3H]quinuclidinyl benzilate binding sites has been demonstrated in cardiac tissue taken from the cat and rat. A similar distribution of both alpha 1- and alpha 2-adrenoceptor sites was seen but the distribution of muscarinic acetylcholine sites was markedly different. alpha-Adrenoceptors were present predominantly in ventricular muscle whereas muscarinic acetylcholine receptors exhibited a greater density in atrial tissue compared to ventricular muscle. Opiate receptors were absent from cardiac tissue.