Dashwood M R, Gilbey M P, Spyer K M
Neuroscience. 1985 Jun;15(2):537-51. doi: 10.1016/0306-4522(85)90232-5.
The distribution of adrenoceptors and opiate receptors in the nucleus of the tractus solitarius and the intermediolateral cell column of the thoracic spinal cord of the cat have been investigated using an in vitro autoradiographic technique. Specific binding of [3H]yohimbine and [3H]rauwolscine (alpha 2-adrenoceptor ligands) was seen within the intermediolateral cell column but no obvious binding of [3H]prazosin, an alpha 1-ligand, was observed. No evidence of a significant population of opiate receptors was obtained in the intermediolateral cell column. Within the nucleus of the tractus solitarius a marked binding of [3H]yohimbine and [3H]rauwolscine was accompanied, however, by a more restricted binding of [3H]naloxone and [3H]dihydromorphine indicating the presence of both alpha 2-adrenoceptors and opiate receptors. As with the intermediolateral cell column no evidence of [3H]prazosin binding was seen. These observations may have particular relevance for the physiology and pharmacology of cardiovascular control. In the case of the intermediolateral cell column it is consistent with evidence of a catecholamine innervation originating from the brainstem. With regard to the nucleus of the tractus solitarius the location of the receptor groups is discussed in the light of the anatomy and physiology of its afferent innervation.
利用体外放射自显影技术，对猫孤束核和胸段脊髓中间外侧细胞柱中肾上腺素能受体和阿片受体的分布进行了研究。在中间外侧细胞柱内可见[3H]育亨宾和[3H]萝芙木碱（α2-肾上腺素能受体配体）的特异性结合，但未观察到α1-配体[3H]哌唑嗪的明显结合。在中间外侧细胞柱中未获得存在大量阿片受体的证据。然而，在孤束核内，[3H]育亨宾和[3H]萝芙木碱的明显结合伴随着[3H]纳洛酮和[3H]二氢吗啡更局限的结合，表明存在α2-肾上腺素能受体和阿片受体。与中间外侧细胞柱一样，未观察到[3H]哌唑嗪结合的证据。这些观察结果可能与心血管控制的生理学和药理学特别相关。就中间外侧细胞柱而言，这与源自脑干的儿茶酚胺神经支配的证据一致。关于孤束核，根据其传入神经支配的解剖学和生理学对受体群的位置进行了讨论。
