Pammar Chandrashekhar, Nayak Ramakan S, Kotrashetti Vijayalakshmi S, Hosmani Jagadish
Department of Oral Pathology and Microbiology, Maratha Mandal's NG Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
J Cancer Res Ther. 2018 Jul-Sep;14(5):983-988. doi: 10.4103/0973-1482.181186.
To compare immunohistochemical expression of CD34 and CD105 in oral submucous fibrosis (OSF) and normal oral mucosa (NOM) and correlate with clinicopathological features of OSF.
Total of thirty clinically diagnosed and histologically confirmed cases of OSF and 15 NOM were included in the study. Tissues sections were immunostained using CD34 and CD105 antibodies.
Twenty-eight cases (93.33%) were positive for CD34, and only 12 cases (40%) were positive for CD105 in OSF. In NOM all cases were positive for CD34 and only one case was positive for CD105. CD34 and CD105 significantly expressed in moderately advanced OSF when compared to very early, early, and advanced cases. It was found that the microvessel density (MVD) in NOM was significantly higher as compared to OSF and MVD decreased with advanced OSF. MVD was higher in CD34 when compared to CD105. MVD decreased with disease progression in OSF using both the markers.
The role of CD34 in determining the premalignant nature of OSF could not be ascertained, since all endothelial cells were positive for CD34, whereas CD105 appeared to be more specific as it is associated with hypoxia-induced angiogenesis which is occurring in OSF due to hyalinization suggesting, CD105 to be more specific marker to determine neoangiogenesis in OSF. Thus further follow-up study of the cases positive for CD105 is required to determine the true nature of angiogenesis in OSF patients.
比较口腔黏膜下纤维化（OSF）和正常口腔黏膜（NOM）中CD34和CD105的免疫组化表达，并与OSF的临床病理特征相关联。
本研究纳入了30例临床诊断并经组织学证实的OSF病例和15例NOM病例。组织切片用CD34和CD105抗体进行免疫染色。
OSF中28例（93.33%）CD34呈阳性，仅12例（40%）CD105呈阳性。在NOM中，所有病例CD34均呈阳性，仅1例CD105呈阳性。与极早期、早期和晚期病例相比，CD34和CD105在中度进展期OSF中显著表达。发现NOM中的微血管密度（MVD）明显高于OSF，且MVD随OSF进展而降低。CD34的MVD高于CD105。使用两种标志物时，OSF中的MVD均随疾病进展而降低。
由于所有内皮细胞CD34均呈阳性，因此无法确定CD34在确定OSF癌前性质中的作用，而CD105似乎更具特异性，因为它与缺氧诱导的血管生成相关，这种血管生成在OSF中由于透明变性而发生，提示CD105是确定OSF中新血管生成的更特异性标志物。因此，需要对CD105阳性病例进行进一步随访研究，以确定OSF患者血管生成的真实性质。
