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微小RNA-16通过抑制Wnt/β-连环蛋白信号通路在体外和体内抑制口腔鳞状细胞癌肿瘤生长。

MiRNA-16 inhibited oral squamous carcinoma tumor growth in vitro and in vivo via suppressing Wnt/β-catenin signaling pathway.

作者信息

Liu Lijun, Jiang Han, Zhao Jin, Wen Hao

机构信息

Department of Stomatology, Stomatology of Mylike Plastic and Cosmetic Hospital of ChongQing, Chongqing, China.

Department of Oral and Maxillofacial Surgery, School of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang, China,

出版信息

Onco Targets Ther. 2018 Aug 23;11:5111-5119. doi: 10.2147/OTT.S153888. eCollection 2018.

DOI:10.2147/OTT.S153888
PMID:30197522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6112799/
Abstract

BACKGROUND

Oral carcinoma, one of the most commonly diagnosed cancers, has a poor prognosis and low survival rate with treatment. In recent years, some studies reported the upregulation of miRNA-16 (miR-16) in the oral carcinoma, whereas some other studies confirmed the downregulation of miR-16. In the current study, we aimed to investigate the function of miR-16 in oral carcinoma.

MATERIALS AND METHODS

Cell proliferation assay was measured by MTT assay, quantitative real time polymerase chain reaction (qRT-PCR) was used to evaluate the expression of miR-16, and apoptosis was analyzed by flow cytometry. In addition, the expression of proteins was detected by Western blot. Moreover, xenograft tumor model was established to detect the effect of miR-16 in vivo.

RESULTS

The results suggested that miR-16 was downregulated in the oral carcinoma tissues. Overexpression of miR-16 inhibited the growth and proliferation of oral squamous carcinoma cells (OSCCs) and induced apoptosis both in vitro and in vivo, which is due to the suppression of Wnt/β-catenin signaling pathway.

CONCLUSION

This study provides evidence that overexpression of miR-16 inhibits OSCC growth by regulating Wnt/β-catenin signaling. Our findings suggest that overexpression of miR-16 could be a potential approach for gene therapy of OSCC in future.

摘要

背景

口腔癌是最常被诊断出的癌症之一，其预后较差，治疗后的生存率较低。近年来，一些研究报道口腔癌中miRNA-16（miR-16）表达上调，而其他一些研究则证实miR-16表达下调。在本研究中，我们旨在探究miR-16在口腔癌中的功能。

材料与方法

通过MTT法检测细胞增殖，采用定量实时聚合酶链反应（qRT-PCR）评估miR-16的表达，并通过流式细胞术分析细胞凋亡。此外，通过蛋白质印迹法检测蛋白质表达。而且，建立异种移植肿瘤模型以检测miR-16在体内的作用。

结果

结果表明，miR-16在口腔癌组织中表达下调。miR-16的过表达在体外和体内均抑制口腔鳞状癌细胞（OSCC）的生长和增殖并诱导细胞凋亡，这是由于Wnt/β-连环蛋白信号通路受到抑制。

结论

本研究提供了证据表明miR-16的过表达通过调节Wnt/β-连环蛋白信号通路抑制OSCC生长。我们的研究结果表明，miR-16的过表达可能是未来OSCC基因治疗的一种潜在方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/86c93bf2e49f/ott-11-5111Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/c90bf7581d03/ott-11-5111Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/3dc165f47904/ott-11-5111Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/77902c310629/ott-11-5111Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/85b3e60bedf3/ott-11-5111Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/2a32722749ef/ott-11-5111Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/86c93bf2e49f/ott-11-5111Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/c90bf7581d03/ott-11-5111Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/3dc165f47904/ott-11-5111Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/77902c310629/ott-11-5111Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/85b3e60bedf3/ott-11-5111Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/2a32722749ef/ott-11-5111Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2917/6112799/86c93bf2e49f/ott-11-5111Fig6.jpg

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本文引用的文献

1
Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer.miR-196b异常的DNA低甲基化促进口腔癌的迁移和侵袭。
Oncol Lett. 2016 Jun;11(6):4013-4021. doi: 10.3892/ol.2016.4491. Epub 2016 Apr 25.
2
miRNAs Signature in Head and Neck Squamous Cell Carcinoma Metastasis: A Literature Review.头颈部鳞状细胞癌转移中的微小RNA特征：文献综述
J Dent (Shiraz). 2016 Jun;17(2):71-83.
3
Clinical development of gene therapy: results and lessons from recent successes.基因治疗的临床发展：近期成功的结果和经验教训。
Review of Disease-Specific microRNAs by Strategically Bridging Genetics and Epigenetics in Oral Squamous Cell Carcinoma.
口腔鳞状细胞癌中通过遗传与表观遗传的策略性桥接来综述疾病特异性 microRNAs
Genes (Basel). 2023 Aug 2;14(8):1578. doi: 10.3390/genes14081578.
4
Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma.唾液miR-30c-5p作为检测口腔鳞状细胞癌的潜在生物标志物
Biomedicines. 2021 Aug 24;9(9):1079. doi: 10.3390/biomedicines9091079.
5
LINC00662 Promotes Oral Squamous Cell Carcinoma Cell Growth and Metastasis through miR-144-3p/EZH2 Axis.LINC00662 通过 miR-144-3p/EZH2 轴促进口腔鳞状细胞癌细胞的生长和转移。
Yonsei Med J. 2021 Jul;62(7):640-649. doi: 10.3349/ymj.2021.62.7.640.
6
LncRNA MORT Inhibits Cancer Cell Proliferation and Promotes Apoptosis in Mantle Cell Lymphoma by Upregulating miRNA-16.长链非编码RNA MORT通过上调miRNA-16抑制套细胞淋巴瘤中的癌细胞增殖并促进细胞凋亡。
Cancer Manag Res. 2020 Mar 23;12:2119-2125. doi: 10.2147/CMAR.S233859. eCollection 2020.
7
The role and mechanism of microRNA‑18a‑5p in oral squamous cell carcinoma.微小 RNA-18a-5p 在口腔鳞状细胞癌中的作用及机制。
Mol Med Rep. 2019 Aug;20(2):1637-1644. doi: 10.3892/mmr.2019.10403. Epub 2019 Jun 20.
8
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Mol Ther Methods Clin Dev. 2016 May 25;3:16034. doi: 10.1038/mtm.2016.34. eCollection 2016.
4
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J Pathol. 2016 Sep;240(1):38-49. doi: 10.1002/path.4752. Epub 2016 Jul 9.
5
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6
Wnt pathway activation by ADP-ribosylation.通过ADP-核糖基化激活Wnt信号通路。
Nat Commun. 2016 May 3;7:11430. doi: 10.1038/ncomms11430.
7
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Mol Cancer. 2016 Apr 7;15:28. doi: 10.1186/s12943-016-0512-8.
8
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9
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10
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Sci Rep. 2016 Feb 10;6:20968. doi: 10.1038/srep20968.