Methods of determing biokinetics of arsenate accumulation and release in regulated by common environmental factors: Practical implications for enhanced bioremediation.

Only little information is available on combined effects of abiotic environmental factors on algal arsenate ( ) metabolic biokinetics. Herein, we demonstrated the methods of using the Taguchi statistical method to investigate four environmental factors including , nitrate (N), orthophosphate (P) and pH for their combined effects on algal growth and arsenic () uptake but also extracellular adsorption of as well as release from dead algal cells. Results showed that an increase of N facilitated growth and thus was the principal factor for the algal maximum specific growth rate ( ). P was vital to bioconcentration factor (BCF) and partition coefficients (Log ) released from deal algal cells. impacted the extracellular adsorption onto the algal cells, which thereby increased with increasing initial level. The initial pH had an imperative effect on the uptake ( ) and release rate ( ) from the dead cells. Collectively, the condition of low P, high N and alkaline pH level was favorable to accumulation rate of living cells and restrictive to release rate from dead cells of . The obtained information can pave a road for extensive understanding on efficient utilization of bioremediation of algae in practical environment. •Principal factors were identified on metabolic biokinetics by Taguchi method.•High N and pH but low P fasten uptake and reduce efflux from dead cells.• only as the main factor impacted extracellular adsorption on algal cells.